02456nas a2200229 4500008004100000245014500041210006900186260001900255300001400274490000700288520166800295653002501963653002101988653001502009653003402024653002002058100003102078700002302109700002502132700002902157856004002186 2021 eng d00aPharmacognostic Specifications, RP-HPLC Analysis of Chlorogenic Acid Content and Antioxidant Activity of Morus alba Linn. Leaves in Thailand0 aPharmacognostic Specifications RPHPLC Analysis of Chlorogenic Ac cSeptember 2021 a1186-11940 v133 a

Background: Morus alba Linn. leaves have been widely used as herbal medicine with therapeutics and contain chlorogenic acid as a bioactive phenolic compound. Objective: The present study aimed to conduct the pharmacognostic specifications of M. alba leaves and the method validation for quantification of chlorogenic acid content. Materials and Methods: Macroscopic- and microscopic characteristics, physicochemical parameters combined with quantification of chlorogenic acid in M. alba leaves collected from 15 sources throughout Thailand and their antioxidant activity were reported in this study. Results: The physicochemical parameters of M. alba leaves were determined by indicating water content (7.97 ± 0.35%), loss on drying (4.55 ± 0.21%), total ash (14.38 ± 0.25%), acid-insoluble ash (6.21 ± 0.37%), water-extractive value (16.14 ± 0.50%) and ethanol-extractive value (8.61 ± 0.39%). In addition, the chlorogenic acid contents in M. alba leaves were found to be 0.4159 ± 0.1958 g/100g dry weight. The ethanolic leaf extracts exhibited their antioxidant activity with half-maximal inhibitory concentration (IC50) values (326.09–467.55 μg/mL). Conclusion: This study showed the establishment of pharmacognostic study of M. alba leaves and validation of the reversed-phase high-performance liquid chromatography (RPHPLC) quantitative analysis of their chlorogenic acid contents, which are applicable to be a reference for quality control and standardization of M. alba leaves.

10aantioxidant activity10aChlorogenic acid10aMorus alba10aPharmacognostic specification10aQuality control1 aAiyarakanchanakun, Phimkun1 aPalanuvej, Chanida1 aRuangrungsi, Nijsiri1 aPhanumartwiwath, Anuchit uhttps://www.phcogj.com/article/1649