03153nas a2200361 4500008004100000245014900041210006900190260001900259300001400278490000700292520196000299653002002259653001902279653000902298653002302307653001702330653002002347100002802367700002902395700002602424700001702450700002602467700002602493700003502519700002202554700003102576700002002607700004402627700003202671700002402703700002402727856004002751 2021 eng d00aEvaluation of the Anticonvulsant, Anxiolytic, Sedative, and Neuroprotective Activities of Polysaccharides from Mycelium of Two Ganoderma Species0 aEvaluation of the Anticonvulsant Anxiolytic Sedative and Neuropr cSeptember 2021 a1161-11730 v133 a

Background: Ganoderma lucidum has been used as a medicinal mushroom since centuries in East Asia. Recent reports have shown that metabolites isolated from Ganoderma species have shown effects on central nervous system. Objective: To determine the neuroprotective, anticonvulsant, anxiolytic, and sedative effects of Ganoderma sp. and Ganoderma curtisii polysaccharides. Methods: Polysaccharides (Gsp-PS2 or Gc-PS2) were isolated from two Ganoderma mycelia submerged cultures. Acute toxicity effects of Gc-PS2 or Gsp-PS2 on mice were treated orally with doses of 50 - 2000 mg/kg. Anticonvulsant activity was determined using three chemoconvulsants: kainic acid (KA), strychnine, or pentylenetetrazole (PTZ). Anxiolytic-like effects were determined using the elevated plus maze test on mice. GABA release evoked by GC-PS2 or Gsp-PS2 content was determined by HPLC. Neuroprotective effects of Gsp-PS2 or Gc-PS2 were determined by glial activation, histopathological changes, and immunohistochemistry. Results: Gc-PS2 or Gsp-PS2 showed neuroprotective activity by diminishing neuronal death, reducing glial activation and Neu-N expression levels. Gsp-PS2 or Gc-PS2 inhibited convulsions in the KA model. An anxiolytic-like, but not a sedative effect was reported in mice treated with Gc-PS2 or Gsp-PS2. Polysaccharides Gc-PS2 or Gsp-PS2 evoked endogenous GABA release and increased its concentration within the incubation medium. Pretreatment with Gsp-PS2 or Gc-PS2 showed a reduction of the LPSinduced NO production. Gc-PS2 or Gsp-PS2 did not produce toxic effects. Conclusion: Ganoderma sp. or Ganoderma curtisii polysaccharides showed neuroprotective and anticonvulsant activities in animal models. The anticonvulsant activity may involve the GABAergic neurotransmision.

10aa- and b-glucan10aAnticonvulsant10aGABA10aGanoderma curtissi10aGanoderma sp10aNeuroprotective1 aNunez-Urquiza, Veronica1 aVilleda-Hernandez, Juana1 aMontiel-Arcos, Elizur1 aTello, Isaac1 aCampos-Pena, Victoria1 aHerrera-Ruiz, Maribel1 aGutiérrez, María, del Carmen1 aPetricevich, Vera1 aSantana, María, Angélica1 aNavarro, Martha1 aAguilar-Guadarrama, Angélica, Berenice1 aNavarrete-Vázquez, Gabriel1 aPerea-Arango, Irene1 aLeon-Rivera, Ismael uhttps://www.phcogj.com/article/1646