02885nas a2200301 4500008004100000245018700041210006900228260001900297300001400316490000700330520186000337653002402197653003402221653003802255653003402293653003002327100000502357700002002362700002402382700001702406700001702423700002602440700000502466700003402471700001702505700002102522856004002543 2021 eng d00aSingle Factor Effect of Natural Deep Eutectic Solvent Citric Acid-Glucose Based Microwave-Assisted Extraction on Total Polyphenols Content from Mitragyna speciosa Korth. Havil Leaves0 aSingle Factor Effect of Natural Deep Eutectic Solvent Citric Aci cSeptember 2021 a1109-11150 v133 a

Applying green chemistry principles in the exploration of an active secondary metabolite from natural products has continued to experience a significant increase in the last decade, mainly natural deep eutectic solvent (NADES) usage. Mitragyna speciosa (Korth.) Havil (Rubiaceae family) is a native of East Kalimantan, which is traditionally used as an herb to treat headaches and inflammation by drinking boiled water a few leaves. On the other hand, this plant has an addictive effect. This study aims to determine the single factor effect of NADES citric acid–glucose-based microwave-assisted extraction (MAE) to obtain the yield of total polyphenols content (TPC) from M. speciosa leaves. Dried powder of M. speciosa leaves was extracted using NADES citric acid-glucose based MAE method with some different conditions such as NADES (citric acid-glucose) ratio (4:1, 5:1, 6:1 g/g), solid-liquid ratio (10:1, 15:1, 20:1 mL/g), microwave power (90, 270, 450 Watts), and extraction time (15, 20, 25 min). The determination of TPC was performed using Folin-Ciocalteau reagent and gallic acid as a standard with different concentration levels. The absorbance measured using spectrophotometer UV-VIS at 746 nm to obtain a regression formula of Y = 0.0022X–0.00095, where R2 = 0.9977. Based on the result, the best of single factor condition effect was obtained the TPC value including NADES ratio of 248.69 mg GAE/g sample (5:1 g/g citric acid-glucose), a solid-liquid ratio of 146.93 mg GAE/g sample (15:1 mL/g solvent-sample), microwave power of 192.20 mg GAE/g sample (270 Watts), and extraction time of 358.59 mg GAE/g sample (15 min). The single factor effect of NADES citric acid-glucose-based MAE shows a difference in TPC value based on various conditions of this method.

10aCitric acid-glucose10aMicrowave-assisted extraction10aMitragyna speciosa (Korth.) Havil10aNatural deep eutectic solvent10aTotal polyphenols content1 a1 aIbrahim, Arsyik1 aRahayu, Bakti, Puji1 aArifuddin, M1 aNur, Yuspian1 aPrabowo, Wisnu, Cahyo1 a1 aAmbarwati, Neneng, Siti Silfi1 aRijai, Laode1 aAhmad, Islamudin uhttps://www.phcogj.com/article/1639