02731nas a2200205 4500008004100000245014700041210006900188260001400257300001400271490000700285520200800292653002602300653002102326653002602347100003502373700003402408700002402442700001902466856004002485 2021 eng d00aChemical Composition and In Vitro Antiplasmodial Activity of the Total Alkaloids of the Bulbs of Two Amaryllidaceae Species from Northern Peru0 aChemical Composition and In Vitro Antiplasmodial Activity of the cJuly 2021 a1046-10520 v133 a

Introduction: The amaryllidaceae family is characterized by presenting alkaloids with powerful pharmacological activities, including antiprotozoal activity. The aim of the present work was to determine the chemical composition and evaluate the in vitro antiplasmodial activity of the total alkaloids of the bulbs of two amaryllidaceae species from northern Perú. Methods: The total alkaloids were extracted from the bulbs using an acid-base extraction. The chemical composition of the total alkaloids was determined by GC-MS, using galantamine as a reference standard. It was investigated the in vitro antiplasmodial activity against Plasmodium falciparum FCR-3 strain (chloroquine-resistant). Results: 8 alkaloids were identified in the bulbs of Clinanthus incarnatus: lycorine, galanthamine, galanthine, vittatine/crinine, hippamine, 3-O-acetylpowelline, 11,12-dehydroanhydrolycorine, 1-O-acetyllycorine with values of 19.73; 14.99; 10.36; 10.22; 10.16; 10.14; 10.04; 9.85 μg GAL/100 mg of total alkaloid (TA) respectively and 6 alkaloids in the bulbs of Clinanthus ruber: lycorine, anhydrolycorine, 11,12-dehydroanhydrolycorine, 2,4-didehydro-2-dehydroxylycorine, 8-0-dimethylmaritidine, hippamine, with values of 70.2; 18; 4.15; 3.45; 6.8 and 0.1 μg GAL/100 mg TA respectively. The total alkaloids of the species of C. incarnatus and C. ruber at concentrations of 1.0; 2.5; 5.0; 10.0; 25.0 and 50.0 μg/ml presented inhibition percentages of 23.5 ± 0.46% to 94 ± 0.56% against P. falciparum with (p <0.05). They also presented IC50 0.375 μg/ml (C. incarnatus) and IC50 0.241 μg / ml (C. ruber). Conclusion: The main component of total alkaloids of the bulbs of two species was lycorine, in adittion, these species showed in vitro antiplasmoidal activity against Plasmodium falciparum FCR-3 strain at the doses tested.

10aClinanthus incarnatus10aClinanthus ruber10aPlasmodium falciparum1 aSoto-Vásquez, Marilú, Roxana1 aPinedo, Madeleine, Vanessa Ho1 aTallini, Luciana, R1 aBastida, Jaume uhttps://www.phcogj.com/article/1630