02091nas a2200229 4500008004100000245007100041210006900112260001400181300001200195490000600207520145400213653001201667653001901679653001001698653001401708653001401722100002501736700001801761700002501779700001801804856003901822 2016 eng d00aIsolation of flavonoid from Abies webbiana leaves and its activity0 aIsolation of flavonoid from Abies webbiana leaves and its activi cJune/2016 a341-3450 v83 a

Background: Abies webbiana commonly known as Talispatra in Bengali and Hindi, Talispatram in Sanskrit and Indian Silver Fir in English. This is a large, tall, evergreen tree occurring in the Himalayan region from Kashmir to Assam in India. It comes under the Family: Pinaceae. The present study was designed for isolation of flavonoid from ethyl acetate extract of A. webbiana leaves and assessed their toxic effect on liver and kidney. Materials and Methods: The isolation of flavonoid using different chromatographic methods (thin layer and column chromatography). The isolated flavonoid was identified; Structures and chemical bonds were analyzed by using MP, FTIR, 1-H NMR and MS spectral analysis. Effect of flavonoid on liver and kidney was assessed by inducing (0.1 ml/kg) CCl4 (i.p.) and (6 mg/kg) Cisplatin (i.p.) respectively measured by biochemical marker of liver and kidney. Results and Discussion: It was identified that isolated compound was as 4’-hydroxy quercetin on the basis of FTIR, 1-H NMR and MS spectral analysis. Isolated flavonoid reduced the increased biochemical marker (BM) of liver and kidney. The BM was increased by inducing CCl4 and Cisplatin respectively. Conclusion: Isolated compound was 4’-methoxy quercetin and significantly protect the liver and kidney.

10a1-H NMR10aAbies webbiana10aCCl4.10aCisplatin10aQuercetin1 aYadav, Dinesh, Kumar1 aAli, Mohammed1 aGhosh, Ashoke, Kumar1 aKumar, Babita uhttps://www.phcogj.com/article/159