02552nas a2200277 4500008004100000245010200041210006900143260001800212300001000230490000600240520172800246653002401974653002201998653001502020653001402035653002702049100001802076700002102094700002102115700002802136700001402164700001802178700001702196700002102213856004002234 2014 eng d00aIsolation of Phytochemicals From Anticancer Active Extracts of Syzygium alternifolium Walp. Leaf0 aIsolation of Phytochemicals From Anticancer Active Extracts of S c26th May 2014 a83-850 v63 a

Objective: The aim of the present study was to isolate the phyto molecules from the leaf of endemic medicinal pant, Syzygium alternifolium. The phytochemical investigation of the leaf of the plant yielded a flavonoid Eucalyptin 1 and a triterpinoid Epibetulinic acid 2 in pure state. Results: The compound 1 is being reported for the first time from this plant. The anti-cancer activity showed leaf hexane extract (IC50 values 8.177 and 2.687 µg/ml) was significantly active, when compared to extracts and compounds, against human cancer cell lines MCF-7 and DU-145. Also, hexane extract potentially inhibited the growth of DU-145 cell lines when compared with the reference compound doxorubicin. Amongst the isolated compounds, 1 was better cytotoxic than 2. Conclusion: The hexane extract of leaves of S. alternifolium yielded compounds 1 and 2 and the structure elucidation, based on spectroscopy, revealed them as Eucalyptin and Epibetulinic acid respectively. The compound 1 is being reported for the first time from this plant. The anti-cancer activity showed leaf hexane extract (IC50 values 8.177 and 2.687 mg/mL) was significantly active, when compared to extracts and compounds, against human cancer cell lines MCF-7 and DU-145. Also, hexane extract potentially inhibited the growth of DU-145 cell lines when compared with the reference compound doxorubicin. Amongst the isolated compounds, 1 was better cytotoxic than 2.

Key Words: Syzygium alternifolium, Myrtaceae, Eucalyptin, Epibetulinic acid, anticancer activity.

10aAnticancer activity10aEpibetulinic acid10aEucalyptin10aMyrtaceae10aSyzygium alternifolium1 aKomuraiah, B.1 aChinde, Srinivas1 aKumar, Niranjana1 aSrinivas, K.V.N., Satya1 aVenu, Ch.1 aKumar, Kotesh1 aSastry, K.P.1 aGrover, Paramjit uhttps://www.phcogj.com/article/1523