02138nas a2200229 4500008004100000245010800041210006900149260001800218300001000236490000600246520147300252653001901725653001301744653000801757653001201765653001401777100001901791700001801810700001801828700002201846856004001868 2014 eng d00aThe In-Vitro Toxic Effect of The Glycoalkaloids for Some Solanum Species Against The LIM-1863 Cell Line0 aInVitro Toxic Effect of The Glycoalkaloids for Some Solanum Spec c26th May 2014 a23-310 v63 a

Background The LIM-1863 Cell Line is one of the colon cancer types considered to be responsible for a high rate of deaths, and the glycoalkaloids being natural substances existing in the Solanum species have anticancer effects. Objective This research aims at studying the effect of the glycoalkaloids on viability of the LIM-1863 cancerous cells in-vitro. Materials and Methods The glycoalkaloids in this study are extracted by the ultrasonic waves technique and detecting them by the Thin Layer Chromatography (TLC) in addition to incubating of the LIM-1863 cells with different concentrations of the glycoalkaloids for 48 hours and then assessing of the cell viability using the MTT assay. Results The findings showed that the glycoalkaloids have a toxic effect on the LIM-1863 cells and that half of the inhibiting concentration (IC50) of the Solanum fruits extract: (Solanum nigrum L.), (Solanum villosum Mill.) and (Solanum elaeagnifolium Cav.) on the LIM-1863 cells have the (164.7, 35.91 and 12.14 μg/ml) values successively. Conclusion The observations indicated that the glycoalkaloids are able to inhibit the colon cancer cell proliferation.

Key words:Solanum, Glycoalkaloids, TLC, LIM-1863, Viability, MTT.

10aGlycoalkaloids10aLIM-186310aMTT10aSolanum10aViability1 aSammani, Ahmad1 aShammaa, Esam1 aChehna, Fawaz1 aRahmo, Abdulkader uhttps://www.phcogj.com/article/1521