02253nas a2200289 4500008004100000245016200041210006900203260001900272300001000291490000600301520133000307653000901637653004001646653002901686653001901715653001401734653002001748653001901768100001901787700002001806700002001826700001801846700002401864700001701888700001801905856004001923 2014 eng d00aAntiproliferative activity of crude extract and fractions obtained from Digera muricata on Hela cell lines of human cervix and A549 cell lines of Human Lung.0 aAntiproliferative activity of crude extract and fractions obtain c3rd Sept, 2014 a32-380 v63 a

Digera muricata (Linn.) of family Amaranthaceae is an ethanobotanically important plant species traditionally used against various disorders. Cytotoxic potential of methanolic extract and its fractions were investigated against Hela and A549 cell lines. Crude extract of Digera muricata was prepared in methanol by Continuous Hot Soxhlation technique. Crude extract was fractionated into two organic and one aqueous fraction by the help of Column Chromatography. MTT assay was used to evaluate the reduction of viability of the cancer cell lines. Cell viability was inhibited by crude extract of Digera muricata in a dose dependent manner ranging from 25μg/ml to 250μg/ml. Apoptosis assays using nucleic acid stains namely PI exclusion assay and Hoestch/PI assay were performed by the help of fluorescence microscopy. Morphological analysis was done by calculation of Apoptotic ratio and Percentage apoptosis. Our results suggests that methanolic and aqueous fraction of the extract of Digera muricata can be good source of cytotoxic compounds.

Key words: 3-(4,5-dimethylthiazol-2yl)-2,4 diphenyltetrazolium bromide assay, A549 cell line, cytotoxic, Digera muricata, HeLa cell line.

10a3-(410a4 diphenyltetrazolium bromide assay10a5-dimethylthiazol-2yl)-210aA549 Cell Line10aCytotoxic10aDigera muricata10aHeLa cell line1 aUsmani, Shazia1 aHussain, Arshad1 aFarooqui, A.H.A1 a.Arshad, Mohd1 aSiddiqui, Sahabjada1 a.Ahmad, Mohd1 aWahab, Shadma uhttps://www.phcogj.com/article/1500