02434nas a2200241 4500008004100000245014100041210006900182260001900251300001200270490000600282520164400288653001701932653002401949653001301973653004201986100001702028700002102045700001602066700002002082700002302102700002702125856004002152 2015 eng d00aInhibition on Urease and Thermal Induced Protein Denaturation of commonly used Antiulcer Herbal Products. Study based on in-vitro assays0 aInhibition on Urease and Thermal Induced Protein Denaturation of c16th Jan, 2015 a147-1510 v73 a

Background: In-vitro urease inhibitory and thermal induced protein denaturation inhibitory activitieswasperformed for two commonly used herbal productsEndemali and Akseer ULCER in the treatment of ulcers.Objectives: to evaluate the antiulcer potential of two commonly used herbal products, Endemali, Akseer ULCER. Material and Method: In urease inhibitory assay , enzyme solution, extract, diferentregaents added and absorbance was measured at 630 nm (50 min, pH 8.2) and thiourea used as standard. In protein denaturation assay the egg albumin was mixed with different concentration of test compounds, buffer absorbance was measured. Aspirin was used as standard. Results: The Endemalihad a profound effect on the urease activity in a concentration dependent manner with EC50 valueof 0.468 mg/ml. The Akseer ULCER antagonized the urease activity markedly with EC50 value of 0.374 mg/ml.These tested herbal products caused marked inhibition of thermal induced protein denaturation in a concentration dependent manner. The potency in the form of EC50 for Endemali, Akseer ULCER was measured as 323, 337 µg/mlrespectively. Conclusion: In short, the tested herbal drug showed strong inhibition on urease activity and inhibition on thermal induced protein denaturation thus our study validated their uses in the treatment of ulcers.

Key words: Akseer ULCER, Endemali, Antiurease activity, Thermal induced protein denaturation.

10aAkseer ULCER10aAntiurease activity10aEndemali10aThermal induced protein denaturation.1 aKhan, Haroon1 aKhan, Murad, Ali1 aRauf, Abdul1 aHaleemi, Ashhad1 aFuloria, Shivkanya1 aFuloria, Neeraj, Kumar uhttps://www.phcogj.com/article/1473