02349nas a2200241 4500008004100000245015000041210006900191260001900260300001000279490000600289520155300295653002101848653001701869653001801886653001201904653001301916100003301929700002101962700003201983700003002015700002202045856004002067 2015 eng d00aEvaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.0 aEvaluation of cytotoxic DNA protecting and LPS induced MMP9 down c27th Nov, 2014 a32-360 v73 a

Introduction:Plectranthus amboinicus (Lour) Spreng is a known medicinal plant used in Siddha and Ayurveda medicines in India. It has enormous medicinal potential to treat various diseases. Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthus amboinicus to test cytotoxicity against breast (MCF-7) and colorectal (HT-29) cancer cell lines, to protect DNA from H2O2 induced genotoxicity through comet assay and to treat inflammation in lipopolysaccharide (LPS) induced over expression of matrix metalloproteinase-9 (MMP-9) in human peripheral blood mononuclear cells (PBMCs) by gelatin zymogram and inhibition at transcriptional level confirmed using RT-PCR (reverse transcriptase polymerase chain reaction). Results: Cytotoxicity of essential oil against MCF-7 and HT-29 cancer cell lines revealed the IC50 values of 53 ± 0.01 and 87 ± 0.01μg/mL respectively. At 200 μg/mL essential oil protected against 75% of DNA damage in 3T3-L1 fibroblast cells. Essential oil showed significant reduction in production of MMP-9 in a concentration dependent manner. Conclusion: Overall, the results showed that essential oil of P. amboinicus is a potent bioactive substance and it could be used in herbal medicine preparations.
Key words: Essential oil , Cytotoxicity , Antigenotoxicity, Zymogram, RT- PCR.

10aAntigenotoxicity10aCytotoxicity10aEssential oil10aRT- PCR10aZymogram1 aThirugnanasampandan, Ramaraj1 aRamya, Gunasekar1 aGogulramnath, Madhusudhanan1 aJayakumar, Rajarajeswaran1 aKanthimathi, M.S. uhttps://www.phcogj.com/article/1458