02197nas a2200229 4500008004100000245008700041210006900128260001800197300001200215490000600227520147800233653002601711653002101737653001901758653002301777653001701800100002701817700002801844700002401872700003201896856003901928 2015 eng d00aAntimicrobial Effects of Thymoquinone on Entamoeba histolytica and Giardia lamblia0 aAntimicrobial Effects of Thymoquinone on Entamoeba histolytica a cDecember 2015 a168-1700 v83 a

Background: Parasitic infections are a major difficulty in tropical and subtropical countries. Traditionally medicinal plants have been used in folk medicine to treat parasitic infections and are a valuable source of novel anti-parasitics. Objective: In our search for therapeutic alternatives to anti- protozoal chemotherapy, thymoquinone, the active ingredient of Black cumin (Nigella sativa) was examined. Materials and Methods: Thymoquinone was tested against Entamoeba histolytica and Giardia lamblia using in vitro susceptibility assays and the mortality of the parasites were then obtained using the standard calculations. The compound was also tested for 48 and 72 hours on both parasites. Results: The current study indicate that the mortality of TQ showed 85.5%, 91.5% and 96.8% mortality on E. histolytica for 25 ppm at 24 hr, 48 and 72 hr, respectively, with IC50 2¥10-19,. On the other hand, this natural compound showed a mortality of 82.83%, 91.76% and 96.62% mortality on G. lamblia for 25 ppm at 24 hr, 48 and 72 hr, respectively, with IC50 4.8¥10-5. Metrondizole powder gave 70.9% mortality at 156 ppm at the same times. Conclusion: The current results indicate that TQ is more potent on E. histolytica compared to G. lamblia. Further pharmacological studies were needed to help in the clinical presentation of thymoquinone.

10aEntamoeba histolytica10aGiardia lamblia.10aNigella sativa10aProphetic Medicine10aThymoquinone1 aSheikh, Bassem, Yousef1 aTaha, Manal, Mohamed El1 aKoko, Waleed, Syaed1 aAbdelwahab, Siddig, Ibrahim uhttps://www.phcogj.com/article/145