02428nas a2200253 4500008004100000245012700041210006900168260001300237300001200250490000700262520163600269653002201905653002301927653002101950653001301971653002201984653001002006100002402016700002802040700001802068700002402086700002402110856004002134 2021 eng d00aQualitative and Quantitative Analysis of 70% Ethanol Extract from Ruta angustifolia for Developing Anti-Hepatitis C Agents0 aQualitative and Quantitative Analysis of 70 Ethanol Extract from cMay 2021 a682-6870 v133 a

Background: Medicinal plants are potential sources for drug candidates. It possesses with various metabolites which have many pharmacology effects. Ruta angustifolia is one of medicinal plants that has been used traditionally for liver disease. Previous study it has been demonstrated to inhibit hepatitis C virus under in vitro cell culture. It decreased protein NS3 level and gave synergistic effect in combination with simeprevir and telaprevir. This plant provides a prospective candidate to develop as anti-HCV Objective: This study evaluates the phytochemistry screening for qualitative assay and determine the concentration of rutin as marker compound for developing R. angustifolia extract as anti-HCV agent. Materials and Methods: R. angustifolia leaves were extracted with 70% of ethanol. Extract and rutin were analysis their anti-HCV activity by in vitro culture cells of Huh7it. The concentration of rutin was determine by TLC densitometry. Results: The 70% ethanol extract of R. angustifolia dan rutin exhibit anti-HCV activities with IC50 value of 2.9 ± 0.8 μg/ml and 28.1 ± 5.6 μg/ml, respectively. Screening phytochemistry demonstrated to contain flavonoid, terpenoid, alkaloid and polyphenols. TLC densitometry analysis yield the concentration of rutin in extract 0.06 %. Conclusion: Extract of 70% ethanol of R. angustifolia has a potential anti-HCV activity. Extract of R. angustifolia may provide a good candidate for developing anti-HCV agents.

10aHepatitis C Virus10aInfectious disease10aMedicinal plants10aMedicine10aRuta angustifolia10aRutin1 aWahyuni, Tutik, Sri1 aPermanasari, Adita, Ayu1 aTumewu, Lidya1 aWidyawaruyanti, Aty1 aHafid, Achmad, Fuad uhttps://www.phcogj.com/article/1412