02423nas a2200313 4500008004100000245011400041210006900155260001500224300001200239490000700251520144600258653002001704653002301724653001101747653001501758100002901773700001901802700002501821700002201846700002101868700003001889700002401919700002801943700001801971700003201989700002502021700002302046856004002069 2021 eng d00aNovel Antiviral Investigation of Annona squamosa Leaf Extract against the Dengue Virus Type-2: In vitro Study0 aNovel Antiviral Investigation of Annona squamosa Leaf Extract ag cMarch 2021 a456-4620 v133 a
Introduction: Dengue virus (DENV) infection is general mosquito-transmitted viral taint. It can lead to the dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Dengue is a solemn illness with no endowed antiviral medication or recognized vaccine. Therefore, we aimed to investigate the activity of Annona squamosa leaf extract (ASLE) against dengue virus type-2 (DENV-2) isolated from Surabaya, Indonesia in 2013 (NCBI accession number: KT012509). Methods: In this study, the antiviral activity of ASLE was evaluated against DENV- 2 in Vero cells using Viral ToxGlo™ Assay. In addition, we used CellTiter-Glo® Luminescent Cell Viability Assay to set the amount of viable cells in culture based on quantitation of the ATP. Results: DENV-2 replication inhibited by ASLE in Vero cells with IC50 = 73.78 μg/mL and SI = 4.49 when cells were treated two days after virus infection, whereas its CC50 for cytotoxicity to Vero cells was 331.54 μg/mL. Interestingly, this is the first report on the investigation of ASLE against DENV-2. Conclusion: In summary, ASLE demonstrated the antiviral activity against DENV-2 with less toxicity, and high possibility as a drug candidate. Therefore, it might be suggested for in vivo assessment in the progress of a potent antiviral against DENV-2.
10aAnnona squamosa10aAntiviral activity10aDENV-210aVero cells1 aAnsori, Arif, Nur Muhamm1 aFadholly, Amaq1 aProboningrat, Annise1 aAntonius, Yulanda1 aHayaza, Suhailah1 aSusilo, Raden, Joko Kunco1 aInayatillah, Bilqis1 aSibero, Mada, Triandala1 aNaw, Sin, War1 aPosa, Gabrielle, Ann Villar1 aSucipto, Teguh, Hari1 aSoegijanto, Soegen uhttps://www.phcogj.com/article/1382