02039nas a2200217 4500008004100000245012100041210006900162260001500231300001200246490000700258520135100265653002501616653002601641653002001667653003101687653001301718100001901731700001301750700001801763856004001781 2021 eng d00aCharacterization and Acute Toxicity Bioactive Compound Canning Waste of Lemuru Fish Oil as Potential Immunomodulator0 aCharacterization and Acute Toxicity Bioactive Compound Canning W cMarch 2021 a376-3820 v133 a

Background: Poly Unsaturated Fatty Acids have beneficial effects as an immunomodulator for periodontitis therapy. Lipid studies show that fish can be a unique source of polyunsaturated fatty acids such as eicosapentaenoic acid and docosahexaenoic acid. Lemuru fish (Sardinella longicep) is one of fish that abbundant in Indonesia. Purpose: This study aims to find out the characterization and acute toxicity of lemuru fish oil for using in dental treatment. Methods: characterization study was tested using capillary gas chromatography coupled method with flame ionization. Acute toxicity performed by given lemuru orally with dose 174.1-917.3mg/20g mice weight for 24 hours observation and sacrificed for histopathology, meanwhile groups 96 hours observation continued. Results: Lemuru Fish oil contains 14.5% eicosapentaenoic acid and 13,2% Docosahexaenoic Acid. Acute toxicity showed the percentage of death below 50%, however, liver structure start to show an effect on dose 355.3mg/20gr Conclusion: Lemuru Fish oil had a component Poly Unsaturated Fatty Acids an potential immunomodulator. There was no acute toxicity found and the maximum dose without changing the microscopic structure of the liver is 174.1mg / 20gr.

10aDocosahexaenoic acid10aEicosapentaenoic acid10aLemuru fish oil10aPolyunsaturated fatty acid10aToxicity1 aDW, Damaiyanti1 aRP, Sari1 aD, Muwarmanti uhttps://www.phcogj.com/article/1372