02089nas a2200241 4500008004100000245016600041210006900207260001500276300001200291490000700303520129200310653001901602653002301621653003301644653001701677653001401694653000901708100002201717700002401739700002501763700001901788856004001807 2021 eng d00aCytotoxicity Study of Ethanol Extract of Bintangor Leaf (Calophyllum soulattri Burm.f) on T47D Breast Cancer Cell Line (Cytotoxicity Study with MTT Assay Method)0 aCytotoxicity Study of Ethanol Extract of Bintangor Leaf Calophyl cMarch 2021 a362-3670 v133 a

Introduction: The public has used Bintangor leaf (Calophyllum soulattri Burm.f) for various medical treatments, including treated inflamed eyes and gout. Aim: This research aimed to determine the cytotoxic effect of ethanol extract and fraction of Calophyllum soulattri Burm. f leaf toward T47D breast cancer cell. Methods: The test used T47D breast cancer cells, the 3-4,5-dimethylthiazol-2yl -2,5-diphenyltetrazolium bromide (MTT) test method, and ELISA Reader to determine the absorbance. This method's principle was the presence of tetrazolium salts by the reductase system in the mitochondria of living cells formed purple formazan crystals. The used parameter was the value of IC50. Results: The result showed that ethanol extract, n-hexane fraction, ethyl acetate fraction, and butanol fraction did not have a cytotoxic effect on T47D breast cancer cell. The values of IC50 respectively are 585.31 μg/ml; 409.33 μg/ ml; 534.08 μg/ml; and 563.22 μg/ml. Conclusion: Ethanol extract and Calophyllum soulattri Burm.f leaf fraction did not have a cytotoxic effect on T47D breast cancer cells.

10aBintangor Leaf10aBreast Cancer Line10aCalophyllum soulattri Burm.f10aCytotoxicity10aMTT Assay10aT47D1 aHusni, Elidahanum1 aWahyuni, Fatma, Sri1 aFitri, Hanifa, Nurul1 aBadriyya, Elsa uhttps://www.phcogj.com/article/1370