02396nas a2200205 4500008004100000245009200041210006900133260001800202300001200220490000600232520175500238653001601993653003702009653002302046653002402069100002002093700001902113700001902132856003902151 2016 eng d00aBioactivity Guided Fractionation of Ethanol Extract of Caesalpinia digyna Rottler Roots0 aBioactivity Guided Fractionation of Ethanol Extract of Caesalpin cDecember 2015 a165-1670 v83 a

Background: Authors have reported earlier that ethanol extract of Caesalpinia digyna Rottler roots exhibits significant antianxiety activity at 400 mg/kg, po, in mice using elevated plus-maze (EPM). Objective: Aim of the study was to isolate antianxiety principle(s) from ethanol extract of C. digyna roots following bioactivity guided fractionation approach. Materials and Methods: Bioactive ethanol extract was partitioned with ethyl acetate to get ethyl acetate soluble (EASF) and ethyl acetate insoluble (EAIF) fractions. A compound (CD1) precipitated from EASF. The two fractions and CD1 were evaluated for antianxiety activity in mice. Column chromatography of EASF yielded 5 fractions (F1-F5), all of which were evaluated for antianxiety activity using EPM. Results: Present study revealed that EASF (80 mg/kg) and CD1 (40 mg/kg) exhibited significant antianxiety activity, while EAIF does not. Among the five fractions, only F4 (40 mg/kg, po), exhibited significant antianxiety activity, which was statistically comparable to that of diazepam (2 mg/kg). Conclusion: Present investigation reveals that EASF obtained by partitioning of ethanol extract of C. digyna roots with ethyl acetate, and a compound CD1, isolated from EASF, exhibit significant antianxiety activity. Among 5 fractions (F1-F5) obtained from column chromatography of EASF, only F4 exhibited significant antianxiety activity. F4 is being processed further to isolate the anxiolytic constituent(s), and CD1 is being characterized.

10aAntianxiety10aBioactivity-guided fractionation10aCaesalpinia digyna10aElevated plus-maze.1 aSingh, Jitender1 aKumar, Ashwani1 aSharma, Anupam uhttps://www.phcogj.com/article/134