02967nas a2200241 4500008004100000245014000041210006900181260001800250300001400268490000700282520218300289653002502472653002302497653001702520653002002537653001802557100001802575700002102593700002602614700002102640700002402661856004002685 2020 eng d00aGC-MS Analysis of Anti-Enterobacterial Dichloromethane Fraction of Mandukaparni (Hydrocotyle javanica Thunb.) – A plant from Ayurveda0 aGCMS Analysis of AntiEnterobacterial Dichloromethane Fraction of cNovember 2020 a1494-15030 v123 a

Background: Mandukaparni (Hydrocotyle javanica Thunb.) is a well known medicinal herb used as folklore medcine in many chronic and infectious gastric and other diseases by the people of Estern Himalayan regions. However, the therapeutic active principles of this plant remained unknown. Objective: The main objective of the study was to characterize antienterobacterial dichloromethane fraction of the volatile oils of it by GC-MS. Materials and Methods: In the present study, dichloromethane (DCM) fraction (MP-DCMf) of Mandukaparni was collected by phase separation of the methanol extract and tested for anti-enterobacterial potentiality against human pathogenic gastrointestinal and food poisoning bacteria by agar well diffusion assay, viability assay and LDH assay and SEM studies. Characterization of the active MP-DCMf fraction was performed by TLC and GC-MS analysis. Results: The MP-DCMf possessed bio-active compounds that have antibacterial potentiality against both the Grampositive and Gram-negative bacteria. The MIC and MBC values were in the range from 1.56 mg/ml to 0.78 mg/ml and 6.25 to 1.56 mg/ml, respectively. The time kill assay showed that at a dose of 3.12 mg/ml of MP-DCMf was lethal to the E. coli MTCC 723 at the 18th hr of treatment. LDH release had moderate positive correlation with the activity index and time of treatment whereas strong negative correlation with CFU count. It caused highest cell disruption in S. mutans. The principal compounds were D-carvon (30.949%);1H-Isoindole-1,3(2H)-dione; 2-(2-chlorophenyl) (28.483%);Cyclohexanone; 2-methyl-5-(1-methylethen) trans (10.04%); D-Limnone (9.256%);2,6-Octadien-1-ol, 3,7-dimethyl- acetate (6.684%); p-Cresol (0.551%), and Thymol (0.118%). Pub-chem database search also supports that these compounds have very strong bactericidal activity by membrane damage as evidenced by LDH and SEM studies. Conclusions: MP-DCMf contains many potential antibacterial compounds that can be used to combat the gastrointestinal and food poising bacterial pathogens.

10aAnti-enterobacterial10aChemical profiling10aMandukaparni10aTime kill assay10aVolatile oils1 aMandal, Manab1 aMisra, Debabrata1 aGhosh, Narendra, Nath1 aMandal, Sukhendu1 aMandal, Vivekananda uhttps://www.phcogj.com/article/1302