02253nas a2200217 4500008004100000245010500041210006900146260001900215300001400234490000700248520158700255653002501842653001701867653001601884653001601900653001701916100001901933700001701952700002601969856004001995 2020 eng d00aOchna kirkii Oliv: Pharmacognostical Evaluation, Phytochemical Screening, and Total Phenolic Content0 aOchna kirkii Oliv Pharmacognostical Evaluation Phytochemical Scr cSeptember 2020 a1317-13240 v123 a

Background: Ochna kirkii Oliv. belongs to family Ochnaceae is commonly known as “mickey mouse plants” in Asia, included in Jakarta. Objectives: This research was conducted to study the pharmacognosy and total phenolic content. Methods: The pharmacognosy study including the macroscopic, microscopic, fluorescence and phytochemical characteristic, which enable to identification of medicinal plant drug, as well as the total phenolic content using spectrophotometric method. Physicochemical parameters, like loss on drying, ash value, extractive values etc. were also determined following WHO guidelines. Results: Physicochemical standard quantified include total ash 5.15% ± 0.4623, acid insoluble ash 0.32% ± 0.2614, water soluble ash 10.72% ± 0.2340, alcohol soluble extractive 12.8% ± 1.3770, ether soluble extractive 7.30% ± 1.8341, water content 5.76 % ± 0.0050, loss on drying 6.91% ± 1.6133. Preliminary phytochemical screening of the leaves showed the presence of flavonoids, tannins, phenols, saponins and triterpenoid. TLC profile of the O.kirkii leaves extracts (n-hexane, DCM, ethyl acetate and 70% ethanol) showed 8, 6, 7 and 10 spots respectively; in different solvent system. The total phenolic content was 252.08 mg GAE/g± 0.4430. Conclusions: The various macroscopic, microscopic, physical and phytochemical analysis of the O.kirkii leaf is useful in standardization for quality, purity and sample identification.

10aChromatogram profile10aFluorescence10aMacroscopic10aMicroscopic10aOchna kirkii1 aHanani, Endang1 aAnggia, Vivi1 aAmalina, Ike, Nurvita uhttps://www.phcogj.com/article/1279