02517nas a2200253 4500008004100000245014700041210006900188260001800257300001200275490000600287520168800293653002101981653002002002653001702022653001402039653002102053100003502074700001902109700001702128700001802145700002802163700003302191856003902224 2016 eng d00aIn vitro Cytotoxicity Studies of Zn (Zinc) Nanoparticles Synthesized from Abutilon indicum L. against Human Cervical Cancer (HeLa) Cell Lines.0 aIn vitro Cytotoxicity Studies of Zn Zinc Nanoparticles Synthesiz cDecember 2015 a127-1310 v83 a

Background: The Zn nanoparticles synthesized from the plant sources are ecofriendly and are potent anticancer agents. Objective: The objective of the present work was to evaluate In vitro cytotoxic activity of Zn nanoparticles green synthezised from Abutilon indicum extract against HeLa cell lines (cervical cancer). Methods: The aqueous extract is prepared by cold extraction (maceration) using water as a solvent. Phytochemical analysis was done by using the standard procedures. Aqueous extract of A. indicum was used for synthesis of Zn nanoparticles. The nanoparticles were characterized by UV-Visible spectrometry and Scanning electron microscopy (SEM) techniques. In vitro cytotoxicity studies of Zn nanoparticles were done by MTT assay using HeLa cell lines. Results: The preliminary phytochemical results revealed that the aqueous extract of A. indicum contains broad spectrum of secondary metabolites like Tannins, Saponins, Glycosides, Flavonoids, Anthroquinones, Terpenoids and Steroids. The U.V spectrophotometeric analysis of Zn nanoparticles displayed maximum absorption at 270 nm and scanning electron microscopic studies showed that the nanoparticles size ranges from 50-500 nm. The MTT assay results revealed that the of Zn nanoparticles exhibits potent cytotoxicity against HeLa cell lines with IC50 value of 45.82 μg/ml. Conclusion: Thus the present study concludes that Zn nanoparticles can be used as a potent drug in alternative therapy for treating the cervical cancer patients.

10aAbutilon indicum10aCervical cancer10aCytotoxicity10aMTT Assay10aZn nanoparticles1 aKulkarni, Badarinath, Druvarao1 aSultana, Samim1 aBora, Mayuri1 aDutta, Ishita1 aPaarakh, Padmaa, Milaap1 aBasappa., Vedamurthy, Ankala uhttps://www.phcogj.com/article/127