01955nas a2200205 4500008004100000245013600041210006900177260001900246300001400265490000700279520127500286653001001561653002201571653002001593653001901613100002601632700002401658700002701682856004001709 2020 eng d00aTotal Phenolic, Total Flavonoid and Phytochemical Screening by FTIR Spectroscopic of Standardized Extract of Mikania micrantha Leaf0 aTotal Phenolic Total Flavonoid and Phytochemical Screening by FT cSeptember 2020 a1395-14010 v123 a

Background: Mikania micrantha is a great plant that has been used as raw material for traditional medicines. Objective: This paper aims to evaluate total phenols, total flavonoids, and phytochemical screening by FTIR spectroscopy of standardized extract of Mikania micrantha leaf to confirm its medicinal values. Materials and Methods: The leaves were extracted by maceration method using ethanol 96% and evaporated by rotary evaporator. The determination of total phenolic and total flavonoid were performed by spectroscopic method. The phytochemical constituent was screened through the bioactive group of the chemical by FTIR analysis. Results: The total phenolic of extract of Mikania micrantha leaf ranged from 13.19±0.74 to 34.24±1.24 mg gallic acid equivalent/g and total flavonoid ranged from 1.11±0.11 to 20.63±0.16 mg quercetin/g. FTIR analysis confirmed the presence of O-H, aliphatic CH, and C=O functional group. Conclusion: the result of this study confirm that Mikania micrantha possesses the potential of bioactive compounds which are responsible for the biological activities that are useful for raw material of traditional medicines.

10aFT-IR10aMikania micrantha10atotal flavonoid10atotal phenolic1 aSumantri, Imam, Bagus1 aWahyuni, Henny, Sri1 aMustanti, Lolyta, Fiti uhttps://www.phcogj.com/article/1259