02482nas a2200217 4500008004100000245010300041210006900144260001600213300001400229490000700243520180000250653001902050653001502069653002502084653000902109653002702118653002102145653003202166100002602198856004002224 2020 eng d00aBioactivity and Extraction Method with Ultrasonication of Nelumbo nucifera Linn. Anti Aging Drinks0 aBioactivity and Extraction Method with Ultrasonication of Nelumb cAugust 2020 a1097-11030 v123 a

Introduction: Nelumbo nucifera Linn. Flowers health drinks currently are one of the popular demand globally in Thailand and all over the world. There are plenty of substandard or over claimed of antioxidant content labeled at health drink bottles. The consumers do not obtain enough health benefits anti-oxidant contents which are destroyed by heat during their production process. Methods: This study aimed to evaluate the optimization of extraction including water, 40 % and 50% ethyl alcohol using maceration and ultra-sonication method to obtain the highest antioxidant activity compared with DPPH, FRAP and ABTS methods were investigated including total phenolic and flavonoid contents by HPLC in different period of time. The analysis of acute toxicity in white wistar rats by oral administrating of Nelumbo nucifera Linn. flowers extract was testing for after 24 h and 14 d. Results: It was found that Nelumbo nucifera Linn. flower extract drink consisted of high contents of gallic acid, catechin and rutin by HPLC method. Nelumbo nucifera Linn flowers Highest Total Phenolic compound in Nelumbo nucifera Linn. flower extracts with sonicating with 50% ethanol was 0.954639 ± 0.109672. Highest Total flavonoids in Nelumbo nucifera Linn. flower extracts macerating in 50 % ethanol at day 5th was 1.100275 ± 0.777271.and the sonication with 40% ethanol was 0.394283+/- 0.51175. Conclusions: The result of acute toxicity analysis showed no toxicity. Nelumbo nucifera Linn. flower extract drinks which are safe as a health drink for consumers. The further market analysis with sensory test should be essential for further research.

10aAcute toxicity10aFlavonoids10aFlower extract drink10aHPLC10aNelumbo nucifera Linn.10aPhenolic content10aUltra sonication Extraction1 aSrichaikul, Buavaroon uhttps://www.phcogj.com/article/1234