02589nas a2200265 4500008004100000245009100041210006900132260001400201300001200215490000700227520178500234653002302019653003202042653002102074653002202095100003302117700002402150700001902174700002102193700002002214700000502234700002002239700002402259856004002283 2020 eng d00aAntimalarial Activity of Flavonoid Compound Isolated from Leaves of Artocarpus altilis0 aAntimalarial Activity of Flavonoid Compound Isolated from Leaves cJune 2020 a835-8420 v123 a

Introduction: Artocarpus altilis leaves extract has previously been reported as a potential antimalarial drug. Inhibition concentration (IC50) against P. falciparum and effective dose values (ED50) against P. berghei have been reported at 1.32 μg/ml and 0.82 mg/kg, respectively. The aim of this study is to identify the active compound from the ethanol extract of A. Altilis leaves against P. falciparum. Materials and Methods: The isolation of the active compound from the ethanol extract of A. altilis were conducted using chromatography methods, and the chemical structure of the isolated compounds was determined based on NMR and MS spectra data. Antimalarial assay was determined using microscopic method against P. falciparum 3D7 and molecular docking studies was performed using Molegro Virtual Docker version 5.5 program. Results: A flavonoid compound, class of dihydrochalcone was finally isolated from A. altilis and identified as 1-(2,4-dihydroxy phenyl)-3-[8-hydroxy-2-methyl-2-(4-methyl-3- pentenyl)-2H-1-benzopyran-5-yl]-1-propanone (Compound-1). Antimalarial activity test revealed that the compound strongly inhibited P. falciparum growth, with IC50 value of 1.05 μM. An in silico study to determine the mechanism of action of the compound revealed the existence a 3.BPF receptor that possesses a cysteine protease inhibitor of falcipain-2. Conclusion: Compound-1 were isolated from the leaves of A. Altilis is a good candidate of new source in the development of antimalarial drugs. An animal study using this compound is recommended before a clinical trial.

10aArtocarpus altilis10aCysteine protease inhibitor10aDihydrochalcones10aP. falciparum 3D71 aHidayati, Agriana, Rosmalina1 aWidyawaruyanti, Aty1 aIlmi, Hilkatul1 aTanjung, Mulyadi1 aWidiandani, Tri1 a1 aSyafruddin, Din1 aHafid, Achmad, Fuad uhttps://www.phcogj.com/article/1184