02045nas a2200205 4500008004100000245009500041210006900136260001300205300001300218490000700231520143500238653001401673653000801687653001301695653001701708653002701725100002901752700001801781856004001799 2020 eng d00aPimpinella Treatment on Reducing Apoptosis of Kidney Cells Following UVB Radiation in Rats0 aPimpinella Treatment on Reducing Apoptosis of Kidney Cells Follo cMay 2020 a503-509 0 v123 a

Introduction: Pimpinella alpina Molk (PM) is a botanical antioxidant was able to inhibit apoptosis in various cells. Apoptosis is a leading cause of tubular atrophy and therefore chronic kidney disease. However, the effect of PM on reducing apoptosis in kidney cells remains unclear. Objective: aim of this study to elucidate the effect of PM on reducing apoptosis in kidney cells. Methods: In the post test only control group design, 35 male rats were grouped into 7 comprise: NC-G, samples were neither exposure to UVB nor PM treatment; NG-7 and NG-15, all samples were only exposure to UVB irradiation for 7 days; P10-7, P15-7, P10-15, P15-15 groups, samples were exposure to UVB for 7 days and treated with PM for 7 and 15 days respectively. Bax and Caspase3 expression were assessed by rt-PCR and IHC staining method. Results: Statistical analysis showed that RNA-Bax and RNA-caspase3, Bax and caspase3 protein expression in P15-7, P10-15 and P15-15 were lower significantly compared to those of NG-7, p<0.05, and no significant difference compared to those of NC-G, p > 0.05. Conclusion: PM treatment with 100 and 150 mg/day for seven and fifteen days were able to decrease Bax and Caspase3 expression in kidney cells following UVB irradiation. Even, the decreased in Bax and caspase3 expression were comparable to normal.

10aApoptosis10aBax10aCaspase310aKidney Cells10aPimpinella alpina Molk1 aNasihun, Taufiqurrachman1 aWidayati, Eni uhttps://www.phcogj.com/article/1139