02474nas a2200277 4500008004100000245011900041210006900160260001300229300001200242490000700254520159600261653001501857653003301872653002601905653002601931653001501957100002501972700002401997700001902021700002802040700001802068700002202086700002402108700002402132856004002156 2020 eng d00aIn vitro Anti-Amebic Activity of Cage Xanthones from Cratoxylum sumatranum Stem Bark Against Entamoeba histolytica0 aIn vitro AntiAmebic Activity of Cage Xanthones from Cratoxylum s cMay 2020 a452-4580 v123 a

Background: Amoebiasis is caused by Entamoeba histolytica, which is a pathogenic species living on human colon tissues. The development of new drugs for anti-amebic are still very needed for clinical treatment. Objective: This aims to identify the compounds in Cratoxylum sumatranum for their anti-amoeba activity. Materials and Methods: In this study we used bioactivity-guided isolation and structural analysis to identified anti-amebic compounds from dichloromethane extract of Cratoxylum sumatranum stem bark. Their anti-amebic activity was determined by an in vitro cell-based assay against Entamoeba histolytica and an enzymatic assay on NAD kinase. Results: Two known compounds from the cage xanthone groups, namely cochinchinoxanthone (1) and cochinchinone D (2), were isolated. The structures of the cage xanthone compounds were established by extensive spectroscopic data analysis. Compound (1) showed the greatest level of anti-amebic activity both in cell-based and enzymatic assay, yielding IC50 values of 4.57 and 12.17 μg/mL, respectively. In contrast, compound (2) yielded IC50 values of 5.19 and 12.60 μg/mL, respectively. Conclusion: When considering the demonstrated anti-amebic activities, it becomes apparent that these compounds, isolated from Cratoxylum sumatranum stem bark, have the potential to be further developed into effective anti-amebic medicine against Entamoeba histolytica.

10aAmoebiasis10aBioactivity-guided isolation10aCratoxylum sumatranum10aEntamoeba histolytica10aNAD kinase1 aWardana, Fendi, Yoga1 aSari, Defi, Kartika1 aAdianti, Myrna1 aPermanasari, Adita, Ayu1 aTumewu, Lidya1 aNozaki, Tomoyoshi1 aWidyawaruyanti, Aty1 aHafid, Achmad, Fuad uhttps://www.phcogj.com/article/1132