02520nas a2200217 4500008004100000245011600041210006900157260001300226300001200239490000700251520181200258653001802070653002102088653001602109653002702125100003002152700002302182700002602205700003102231856004002262 2020 eng d00aStandardization of Indonesian Traditional Antihypertensive Medicines (Jamu) through the ACE Inhibitor Mechanism0 aStandardization of Indonesian Traditional Antihypertensive Medic cMay 2020 a422-4290 v123 a

Introduction: Herbal medicine (jamu) is a traditional Indonesian drug that has been used by the community in efforts to overcome health problems. One of the herbs that are frequently used by the public is antihypertensive jamu. This study aimed to determine the standardization parameters of 8 antihypertensive jamu in the form of specific and nonspecific parameters, antioxidant and angiotensin-converting enzyme inhibitor (ACEI) activity. Materials and methods: Jamu were extracted using ethanol. Nonspecific parameters that are water content, ash content, ash insoluble acid content, level of substances dissolved in alcohol and water, Coliform microbial contamination, and mold/yeast numbers. Determination of specific parameters including determining organoleptic (color and texture), chemical content, identification of infrared spectrum, in-vitro antioxidant activity, and ACE inhibitor activity. Results: nonspecific parameter such is the average water content of 5.92-8.1 v / w; total ash content of 5.85-7.2 w / w, levels of ash insoluble acid content were 0.45-0.55 w/w and the level of substances dissolved in alcohol and water were 24.22-54.21 and 24.22-54,21, respectively. The eight extracts were uncontaminated with coliform, mold, and yeast microbes. Antioxidant and ACE inhibitor activity test showed that all eight extracts had antioxidant activity in vitro with IC50 values ranging from 9.31 - 157.9 ppm and ACE inhibitor activity with the IC50 value is in the range of 18.37-740.8 ppm. Conclusion: The eight antihypertensive jamu met the standard of extract parameters both the specific and nonspecific and have potential in-vitro activities as ACE inhibitors.

10aACE inhibitor10aAntihypertensive10aAntioxidant10aHerbal medicine (jamu)1 aEff, Aprilita, Rina Yanti1 aRahayu, Sri, Teguh1 aMahayasih, Putu, Gita1 aJanuarko, Muhammad, Unggul uhttps://www.phcogj.com/article/1127