02643nas a2200301 4500008004100000245012300041210006900164260001500233300001200248490000700260520171200267653001701979653001601996653001702012653001402029653001502043100001802058700001902076700002102095700003502116700002802151700002302179700002902202700002802231700002102259700002102280856004002301 2020 eng d00aSynthesis, Characterization, and Cytotoxicity Evaluation of Gallic Acid Nanoparticles Towards Breast T47D Cancer Cells0 aSynthesis Characterization and Cytotoxicity Evaluation of Gallic cMarch 2020 a321-3270 v123 a

Introduction: Gallic acid is a naturally polyphenolic acid which shows cytotoxicity against several cancer cells, as well as it displays chemo-preventive activity which is attributed to its strong apoptosis- inducing and antioxidant effects. Thus, gallic acid has become an attractive substance to be further developed due to its strong cytotoxic activity. This study aimed to synthesize gallic acid nanoparticle coating with alginate-chitosan, and evaluate its cytotoxicity against breast T47D cancer cells. Methods: Gallic acid nanoparticle was synthesized using ionic gelation method. The yield, size and morphology of the nanoparticles were determined by UV-Vis Spectroscopy, Transmission electron microscopy (TEM) and Fourier Transform Infrared (FTIR) spectroscopy. Cytotoxicity evaluation of gallic acid nanoparticle towards breast T47D cancer cell is carried out by MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide) assay. Results: Spherical nanoparticles of gallic acid with the size of 100-200 nm has been successfully synthesized in 96% of yield. Compared to gallic acid (IC50: 20.86 μg/mL) and alginate-chitosan nanoparticle (IC50: 38.46 μg/mL), gallic acid coating with alginate-chitosan nanoparticles demonstrated higher cytotoxicity towards breast T47D cancer cells with IC50 value of 9.03μg/mL. Conclusion: Our results clearly confirmed that gallic acid nanoparticles coating with alginate-chitosan showed a strong cytotoxicity towards breast T47D cancer cells, which is potential to be developed as a candidate for new anti-breast cancer agent.

10aCytotoxicity10aGallic acid10aNanoparticle10aSynthesis10aT47D cells1 aArsianti, Ade1 aBahtiar, Anton1 aFadilah, Fadilah1 aWangsaputra, Vincent, Kharisma1 aParamita, Rafika, Indah1 aAzizah, Norma, Nur1 aNadapdap, Lince, Dameria1 aFajrin, Ajeng, Megawati1 aTanimoto, Hiroki1 aKakiuchi, Kiyomi uhttps://www.phcogj.com/article/1108