01688nas a2200193 4500008004100000245006900041210006900110260001500179300001200194490000700206520108900213653002101302653004701323653001301370100002801383700002201411700002101433856004001454 2020 eng d00aProperties of A Thiamine Binding Protein Purified from Mung Bean0 aProperties of A Thiamine Binding Protein Purified from Mung Bean cMarch 2020 a266-2700 v123 a
Thiamine (vitamin B1) was the first B vitamin which has been identified. It serves as a cofactor for several enzymes involved in energy metabolism. The laboratory test against thiamine deficiency can be done by measuring thiamine levels in the blood. The aim of this study was to identify the stability and the binding activity characters of TBP. The equilibrium dialysis technique was used to see the factors affecting the bond between TBP and thiamine. The MBTBP concentration of post-chromatographic affinity resulted from dilution of lyophilisate was stable for 30 days at -20°C and 3 days at 4°C. The optimal pH for binding MBTBP to thiamine was 7.5. Alkylation with iodoacetic acid decreased the binding capacity of TBP which suggested the presence of a–SH or imidazol group in its active site. The importance of disulfide bridge was proven by decreasing of Thiamine binding capacity of TBP after β-mercaptoethanol treatment. This binding activity was also affected by oxidizing agents, but it was less affected by calcium ions and heavy metals.
10aBinding capacity10aMung bean thiamine binding protein (MBTBP)10aThiamine1 aGunarti, Dwirini, Retno1 aKartika, Megawati1 aSadikin, Mohamad uhttps://www.phcogj.com/article/1103