02259nas a2200229 4500008004100000245009400041210006900135260001500204300001200219490000700231520155200238653001201790653004001802653001301842653002501855100001801880700002201898700001801920700002801938700002301966856004001989 2020 eng d00aLeucaena leucocephala (Lam.) de Wit Seeds: A New Potential Source of Sulfhydryl Compounds0 aLeucaena leucocephala Lam de Wit Seeds A New Potential Source of cMarch 2020 a298-3020 v123 a

Background: Leucaena leucocephala (Lam.) de Wit seeds are considered the most widely consumed legumes by ruminants. The seeds contain around 1.5 mmol of thiol/sulfhydryl compounds per 100 g of dried seeds. The contents of the sulfhydryl compounds can act like glutathione. On the other hand, the intake of a high amount of Leucaena leucocephala (Lam.) de Wit seeds is limited by mimosine because it can induce toxicity and death in ruminants. Objective: The aim of this study was to determine sulfhydryl compound levels in Leucaena leucocephala (Lam.) de Wit seeds after the mimosine removal process. Materials and methods: Leucaena leucocephala (Lam.) de Wit seeds were soaked in aquadest for 24 hours and then dried at 40°C for 86 hours. The dried seeds were macerated at room temperature using 30%, 50%, 70%, and 96% (v/v) of ethanol as the solvent with a 1:10 solvent-to-solid ratio. Levels of dried extract yield were determined for sulfhydryl compounds and mimosine using a spectrophotometer. Results: The 30% ethanol extract without soaking produced the highest levels of extract yield, but 70% ethanol was the most effective solvent for extracting the maximum sulfhydryl and minimum mimosine levels. Conclusion: Ethanol solvent (70%) can be used to extract maximum levels of sulfhydryl compound and minimum levels of mimosine from Leucaena leucocephala (Lam.) de Wit soaked seeds.

10aEthanol10aLeucaena leucocephala (Lam.) de Wit10aMimosine10aSulfhydryl compounds1 aWardatun, Sri1 aHarahap, Yahdiana1 aMun'im, Abdul1 aSaputri, Fadlina, Chany1 aSutandyo, Noorwati uhttps://www.phcogj.com/article/1098