03097nas a2200241 4500008004100000245013100041210006900172260001900241300001200260490000700272520229200279653002502571653002602596653002902622653001502651653002702666653003502693100002202728700003102750700001802781700001602799856004002815 2020 eng d00aAntioxidant and Antibacterial Assay Against Fish Pathogen Bacteria of Kjellbergiodendron celebicum (Koord.) Merr. Leaf Extract0 aAntioxidant and Antibacterial Assay Against Fish Pathogen Bacter cFebruary 2020 a173-1790 v123 a

Introduction: Kjellbergiodendron celebicum (Koord.) Merr. (local name: tombe uwa) is a plant endemic to Sulawesi, Indonesia, and grows around lakes or aquatic environments where fish live. Based on phytochemical screening in previous studies, i.e. methanol extract and ethyl acetate fraction from the leaves of Kjellbergiodendron celebicum (Koord.) Merr., the methanol extract gives positive results containing polyphenol compounds in the flavonoid group which have been known to have strong antioxidant and antibacterial properties. Objective: To test the effectiveness of the comparison of the natural content in the compounds (antibacterial and antioxidant properties) and the total content of phenol in Kjellbergiodendron celebicum (Koord.) Merr., which was extracted using two methods, i.e. maceration and Ultrasonic- Assisted Extraction (UAE), in fish-disease bacteria. Method: The leaves were separated to be extracted with two different methods: maceration and Ultrasound-Assisted Extraction (UAE). Extracts were first screened qualitatively for antioxidant activity and then quantified with respect to in vitro antioxidant activity using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. Antibacterial activity was determined by the paper disc diffusion method and microdilution. Results: 70% Ethanol in leaves extract of Kjellbergiodendron celebicum (Koord.) Merr. The extract which has the highest activity based on the DPPH test and FRAP test is the extract from UAE extraction with IC50 value of 9.81512 μg/mL and ferrous equivalent antioxidant capacity (FeEAC) value of 1.661.3 μmol/gr. UAE method also has a higher potential in antibacterial activity based on the diffusion method of paper discs and microdilution with the MIC obtained as much as 390.6 μg/mL. Conclusion: the UAE extraction method is better at scanning polyphenol compounds compared to the conventional maceration extraction method. Therefore, the results of the antioxidant and antibacterial activity using the UAE method are better than the maceration method.

10aAeromonas hydrophila10aEdwardsiella ictaluri10aFlavobacterium columnare10aMaceration10aPhytochemical compound10aUltrasonic-Assisted Extraction1 aPriscilia, Bianca1 aNugraha, Media, Fitri Isma1 aNovita, Hessy1 aElya, Berna uhttps://www.phcogj.com/article/1083