02628nas a2200265 4500008004100000245008100041210006900122260001900191300001200210490000700222520184900229653001502078653002402093653000902117653002602126653002202152653002202174100002302196700002402219700001802243700002202261700001702283700002202300856004002322 2020 eng d00aQualitative and Quantitative Assay of Hydroxycinnamates of Prunus spinosa L.0 aQualitative and Quantitative Assay of Hydroxycinnamates of Prunu cFebruary 2020 a157-1610 v123 a

Background: Blackthorn (Prunus spinosa L.) is a plant commonly found in the Russian Federation on the roadsides, forest margins, and meadows. Despite lack of recognition by the official medicine, blackthorn fruits possess antioxidant properties and are used in homeopathic preparations. They may also demonstrate antibacterial and anticancer potential due to hydroxycinnamic acids. The aim of present study was to identify and assay hydroxycinnamates in fruits of P. spinosa cultivated in Moscow Region. Materials and Methods: Fresh and dried fruits of P. spinosa, gathered from plants cultivated in Moscow Region in the harvest maturity stage, were used in the study. Qualitative composition of hydroxycinnamic acids was assessed by high performance liquid chromatography, using reversed phase C18 column. Total hydroxycinnamic acids (THA) content was assessed spectrophotometrically. Results: Similar chromatographic profiles were obtained for both fresh and dried blackthorn fruits, the two most abundant compounds being epicatechin (2.91%) and chicoric acid (2.90%). Fruits gathered in Chekhovsky District had lower content of hydroxycinnamates (0.798 ± 0.89) than those coming from Klinsky District (0.886 ± 0.92). However, the THA content in dried fruits grown in both districts was found to be similar (0.540 ± 0.71 and 0.557 ± 0.74, respectively). Conclusions: It can be concluded that blackthorn fruits can be considered as a source of hydroxycinnamic acids, as both fresh and dried fruits contain at least eleven hydroxycinnamates. It was found that the dried fruits have similar content of hydroxycinnamic acids, independently of their origin. Future research should be aimed at drying method optimization.

10aBlackthorn10aHerbal raw material10aHPLC10aHydroxycinnamic acids10aPrunus Spinosa L.10aSpectrophotometry1 aKondrashev, Sergey1 aNesterova, Nadezhda1 aLuzin, Alexey1 aKochanov, Vitaliy1 aLuzina, Anna1 aMatyushin, Alexey uhttps://www.phcogj.com/article/1076