03152nas a2200289 4500008004100000245010200041210006900143260001700212300001400229490000700243520225600250653001902506653001202525653001402537653002302551653002302574100002302597700002502620700001802645700002202663700002802685700001802713700003002731700002702761700003402788856004002822 2019 eng d00aAntiplasmodial Activity of Ethanolic Extract of Macaranga Gigantea Leaf and Its Major Constituent0 aAntiplasmodial Activity of Ethanolic Extract of Macaranga Gigant cOctober 2019 a1181-11880 v113 a

Introduction: This research main goal is to study the antiplasmodial activity of Macaranga gigantea leaf ethanolic extract and its major components on malaria parasites using ex vivo model. Methods: This study was conducted by extraction of M. gigantea leaves using ethanol and isolation of its major constituent. The extract and isolate were tested ex vivo on Balb-C mice’s blood after i.p. administration of Plasmodium berghei strain ANKA. Antiplasmodial activity was observed from mice blood treated by various concentration of either extract or isolate and the parasitaemia percentage were determined by calculating infected blood cell after 24 h of the treatment. It is expressed as decreased of parasitaemia levels and percent of inhibition. Qualitative analysis of active fraction were tested by HPLC method. Chemical structure of isolate were characterized by using UV, IR, 1H-NMR, 13C-NMR and MS spectrophotometry. Results: Ex vivo antiplasmodial study gave the percent inhibition as much as 92.1; 85.7; 64.1; 41.5 and 21.7% at extract concentrations of 300, 100, 30, 10 and 3 μg/ mL respectively. The IC50 values of the extract was 27.1 μg/ml. With respect to the percent of inhibition, at the same concentration, the isolate showed activity as much as 70.2; 62.5; 39.1; 21.7 and 10.8%. The IC50 value of the isolate was 60.2 μg/ml. At the same concentration with extract and Isolate, Pyrimethamine as positive control gave percent inhibition of 94; 87.5; 44.8; 15.; and 12%, with IC50 of 31.4 μg/ml. The results showed that major constituent of M. gigantea leaves is flavonoid. HPLC analysis using a photo diode-array detector showed that the active fraction have same retention time with that of apigenin as standard. Based on instrumental analysis data and compared with literature, a flavonoid derivate known as apigenin can be said has been isolated. Conclusion: It can be concluded that either M. gigantea leaves extract or isolated active constituent known as apigenin have potent antiplasmodial property.

10aAntiplasmodial10aEx vivo10aFlavonoid10aMacaranga gigantea10aPlasmodium berghei1 aMuhaimin, Muhaimin1 aYusnaidar, Yusnaidar1 aSyahri, Wilda1 aLatief, Madyawati1 aPutri, Riski, Dwimalida1 aUtami, Andita1 aChaerunisaa, Anis, Yohana1 aAditama, Andreas, Yoga1 aSiregar, Josephine, Elizabeth uhttps://www.phcogj.com/article/1004