@article {1127, title = {Standardization of Indonesian Traditional Antihypertensive Medicines (Jamu) through the ACE Inhibitor Mechanism}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {422-429}, type = {Original Article}, chapter = {422}, abstract = {

Introduction: Herbal medicine (jamu) is a traditional Indonesian drug that has been used by the community in efforts to overcome health problems. One of the herbs that are frequently used by the public is antihypertensive jamu. This study aimed to determine the standardization parameters of 8 antihypertensive jamu in the form of specific and nonspecific parameters, antioxidant and angiotensin-converting enzyme inhibitor (ACEI) activity. Materials and methods: Jamu were extracted using ethanol. Nonspecific parameters that are water content, ash content, ash insoluble acid content, level of substances dissolved in alcohol and water, Coliform microbial contamination, and mold/yeast numbers. Determination of specific parameters including determining organoleptic (color and texture), chemical content, identification of infrared spectrum, in-vitro antioxidant activity, and ACE inhibitor activity. Results: nonspecific parameter such is the average water content of 5.92-8.1 v / w; total ash content of 5.85-7.2 w / w, levels of ash insoluble acid content were 0.45-0.55 w/w and the level of substances dissolved in alcohol and water were 24.22-54.21 and 24.22-54,21, respectively. The eight extracts were uncontaminated with coliform, mold, and yeast microbes. Antioxidant and ACE inhibitor activity test showed that all eight extracts had antioxidant activity in vitro with IC50 values ranging from 9.31 - 157.9 ppm and ACE inhibitor activity with the IC50 value is in the range of 18.37-740.8 ppm. Conclusion: The eight antihypertensive jamu met the standard of extract parameters both the specific and nonspecific and have potential in-vitro activities as ACE inhibitors.

}, keywords = {ACE inhibitor, Antihypertensive, Antioxidant, Herbal medicine (jamu)}, doi = {10.5530/pj.2020.12.65}, author = {Aprilita Rina Yanti Eff and Sri Teguh Rahayu and Putu Gita Mahayasih and Muhammad Unggul Januarko} } @article {267, title = {ACE Inhibitory Activity, Total Phenolic and Flavonoid Content of Pereskia saccharose Griseb. Leaves Extract}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {285-287}, type = {Original Article}, chapter = {285}, abstract = {

Introduction: Angiotensin-converting enzyme inhibitors (ACEi) are drugs that can control hypertension. Pereskia saccharose Griseb. leaves have been used traditionally as antihypertensive. Objective: The objective of this study was to determine the antihypertensive activity through inhibition of ACE activity, the total phenolic content and total flavonoid content of the ethanolic extract of Pereskia saccharose Griseb. leaves and its fractions. Methods: Extraction was done by maceration with 80\% ethanol and fractionation performed by liquid-liquid partition. Results: In vitro ACE inhibitory activity assay of the ethanolic extract using ACE Kit-WST Dojindo had IC50 value of 3.448 \μg/mL and ethyl acetate fraction had IC50 value of 1.714 x 10-3 \μg/mL. Ethyl acetate contained the highest amounts of both TPC (72.991 \± 0.932 mg GAE/g sample) and TFC (61.337 \± 1.612 mg QE/g sample). Conclusion: The results suggest that Pereskia saccharose Griseb. possess ACE inhibitory activity.

}, keywords = {ACE inhibitor, Flavonoid, Pereskia saccharose Griseb, phenolic}, doi = {10.5530/pj.2017.2.49}, url = {http://phcogj.com/fulltext/316}, author = {Sarlina Jihan Lusiyanti and Katrin and Rissyelly and Nuraini Puspitasari and Putu Gita Maya Widyaswari Mahayasih} } @article {260, title = {ACE Inhibitory Activity, Total Phenolic and Flavonoid Content of Watercress (Nasturtium officinale R. Br.) Extract}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {249-251}, type = {Original Article}, chapter = {249}, abstract = {

Introduction: Hypertension is the main risk factor for cardiovascular disease. There are many developed antihypertension drugs, one of them is focusing in ACE (Angiotensin Converting Enzyme) inhibition activity. ACE inhibition activity known can decrease vasoconstriction effect and also can decrease bradykinin degradation (vasodilator) by creating NO (nitric oxide). Methods: In this study, we conducted an in vitro ACE inhibition activity test which was obtained from watercress on 70\% ethanolic extract and each fraction (n-hexane, ethyl acetate, and n-butanol). Results: Results of the study showed that ethanolic extract of watercress had ACE activity with IC50 value was 19.05 g/mL and the highest IC50 of each fraction is ethyl acetate with IC50 value was 2,303 g/ mL. n-butanol fraction had the highest total phenolic content with 15.798 mg GAE/g of the extract, while the highest total flavonoid content was obtained on ethyl acetate fraction with 82.847 mg QE/g of the extract. Conclusion: The results suggest that Watercress (Nasturtium officinale R. Br.) possess ACE inhibitory activity.

}, keywords = {ACE inhibitor, Flavonoid, phenolic, Watercress (Nasturtium officinale R. Br.)}, doi = {10.5530/pj.2017.2.42}, url = {http://phcogj.com/fulltext/309}, author = {Catty Amalia Yaricsha and Rissyelly and Katrin} }