@article {998, title = {Virtual Screening of Indonesian Herbal Database as alpha-Amino-3- Hydroxy-5-Methyl-4 Isoxazolepropionic Acid (AMPA) Antagonist}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1204-1210}, type = {Original Article}, chapter = {1204}, abstract = {

Objective: Ischemic stroke is one type of circulatory disturbance caused by blood clots that block blood flow to the brain. One of the impact of ischemia is nerve cell damage due to excitotoxicity. Inhibition of the ionotropic glutamate receptor such as the AMPA receptor, becomes an essential approach to the treatment of ischemia. This study aims to explore the possibility of an Indonesian herbal compound as an AMPA receptor antagonist. Methods: In this study, virtual screening of 2233 herbal compounds was performed by docking method using AutoDock to find the antagonist candidate of AMPA receptor from Indonesian herbal database. The virtual screening method was validated by an area under curve (AUC) of the ROC curve and enrichment factor (EF). Lipinski{\textquoteright}s Rule of Five was used to filter the screening result. Results: The validation of virtual screening result showed that AUC was 0.9385 and EF 1\% was 23.5550. The screening result of Indonesian herbal database showed top five compound sanggenol O, blazeispirol X, progesterone, nimolicinol and boeravinone F (-8.51; -8.39; -8.19; -8.17; -8.08 kcal/mol, respectively) and have interaction with TYR61 and THR91 residues of AMPA receptor. Conclusion: Five compounds of the Indonesia herbal database were shown as hits of AMPA receptor antagonist based on the docking method.

}, keywords = {AMPA receptor, AutoDock, Herbal plants, Neuroprotective, Virtual Screening}, doi = {10.5530/pj.2019.11.187}, author = {Rezi Riadhi Syahdi and Chindy Dwi Martinah and Arry Yanuar} } @article {249, title = {Investigation of cytotoxicity induced by Nigella sativa and Azadirachta indica using MDA-MB-231, HCT 116 and SHSY5Y cell lines}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {192-195}, type = {Original Article}, chapter = {192}, abstract = {

Background: Indian herbal plants are widely used as medicine in the ancient ayurvedic and culinary purposes. Nigella sativa that also called as in black cumin is a flowering plant in the family ranunculiceae that is native to Southeast Asia. More recently Nigella sativa is also been used as anti cancer drug and protective agent against gamma radiation induced adverse effects in cell lines. Azadirachta indica commonly called as neem, is a tree belonging to mahogany family meliaceae. Azadirachta indica is also a traditional medicinal plant that used from a very long time in Indian ayurvedic and it is also been reported to have many beneficial effects including but not limited to anti-cancer and anti-diabetic effects. Objective: Find the IC50 values of Nigella sativa and Azadirachta indica ethanolic extracts in MDA-MB-231, HCT 116 and SHSY5Y cell lines. Methods: In this study we selected two cancerous cell lines (MDA-MB-231, HCT 116) and one neuronal cell line (SHSY5Y) and studied the effect of the two plant extracts namely Nigella sativa and Azadirachta indica on the cell metabolic activity. Results: This study revealed that cancerous cell lines are more prone to the plant extracts than the neuronal cell lines. These results suggest positive clues on how such medicinal plant extracts act against cancerous cells alone while affecting the normal cells to a limited extent. However, further studies are required to find if this effect is due to cytotoxicity, cytostaticity, or anti-adhesive property.

}, keywords = {Ayurvedic medicine, Cancer cells, Herbal plants, Neuronal cells}, doi = {10.5530/pj.2017.2.31}, url = {http://phcogj.com/fulltext/298}, author = {Sayani Banerjee and Shefali Pandey and Purbasha Mukherjee and Afia Sayeed and Apoorva Vasant Pandurangi and Shinomol George and Sahabudeen Sheik Mohideen} }