@article {1324, title = {Tinospora Sinensis (Lour.) Merr. Stem Modulate The TNF-Alpha Expression In HCT- 116 Tumour Cell, Besides the Inhibitory Effect on Cervical, Colon and Breast Cancer Cell Lines and Mycobacterium Tuberculosis H37Rv}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {January 2021}, pages = {8-16}, type = {Original Article}, chapter = {8}, abstract = {

Background: The present study was designed to evaluate TNF-Alpha experession, anticancer and antitubercular properties for the stem extracts of Tinospora sinensis (TS). Objective: natural product research is widely used for identifying hit molecules for life threatening diseases including cancer, tuberculosis and drug resistant infections. Materials and Methods: There were three polarity dependant solvent extracts obtained through cold maceration process using ethanol (ELTS), ethyl acetate (EATS) and n-hexane (NHTS), respectively. The extracts were subjected to MTT assay for their anticancer potential against HeLa (cervical cancer), MCF-7 (breast cancer) and HCT116 (colon cancer) cell lines, and based on the results, NHTS was subjected to flow cytometry for TNF-Alpha expression in HCT-116 cells. The antitubercular activity for the extracts was performed against Mycobacterium tuberculosis H37Rv (Mtb) by luciferase reporter phage (LPS) assay method. Results: The result of anticancer screening revealed that n-hexane extracts showed the significant inhibition (p\<0.05) on HCT-116 cells with the IC50 of 177.4 μg/ml, whereas EATS and ELTS were equally active on HeLa with the respective IC50 of 236 and 277 μg/ml. The NHTS was significantly effective on decreasing (P\<0.05) TNF-Alpha expression (31.27 MFU) in HCT-116 cells and is closely active with standard simvastatin (26.7 MFU) against the control (7.06 MFU). The antitubercular activity results revealed the equi-potency of both NHTS and EATS on Mtb with growth inhibition of 84 \% at 100μg/ml. The GC-MS analyses of NHTS confirmed the presence of Berberine, palmatine, tembertarine, magniflorine, choline and tinosporin. Conclusion: Overall, we scientifically support the traditional use Tinospora sinensis stem in the treatment of cancer and immune diseases.

}, keywords = {Anticancer, Antitubercular, HCT-116, Immunomodulatory, Tinospora sinensis, TNF-Alpha}, doi = {10.5530/pj.2021.13.2}, author = {Sreelakshmi Bada Venkatappa Gari and Ramalingam Peraman} } @article {218, title = {Cytotoxic Compounds from Kibatalia gitingensis (Elm.) Woodson}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {December 2016}, pages = {8-13}, type = {Original Article}, chapter = {8}, abstract = {

Ursolic acid (1), squalene (2), a mixture of \α-amyrin acetate (3a) and lupeol acetate (3b), and isoscopoletin (4), isolated from the dichloromethane extracts of the leaves and twigs of Kibatalia gitingensis, were evaluated for their cytotoxic activities against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast-neonatal (HDFn), using the in vitro PrestoBlue cell viability assay. Compounds 1-4 exhibited strong cytotoxic activities against HT-29 cells with IC50 values ranging from 0.6931 to 1.083 \μg/mL. Furthermore, 1-4 were moderately cytotoxic against HCT-116 cells with IC50 values ranging from 4.065 to 11.09 \μg/mL. These compounds were least cytotoxic against MCF-7 cells with IC50 values ranging from 8.642 to 25.87 \μg/mL. The most cytotoxic against HT-29 cells, HCT-116 cells and MCF-7 cells are 2, 4 and 1, respectively.

}, keywords = {Apocynaceae, Cytotoxicity, HCT-116, HDFn, HT-29, Isoscopoletin, Kibatalia gitingensis, Lupeol acetate, MCF-7, PrestoBlue{\textregistered} cell viability assay., Squalene, Ursolic acid, α-amyrin acetate}, doi = {10.5530/pj.2017.1.2}, author = {Mariquit M. De Los Reyes and Glenn G. Oyong and Vincent Antonio S. Ng and Chien-Chang Shen and Consolacion Y. Ragasa} }