@article {105, title = {Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {09/2015}, pages = {50-55}, type = {Original Article}, chapter = {50}, abstract = {

An efficient transformation system for the medicinal and aromatic plant, Pogostemon cablin Benth was developed by using Agrobacterium rhizogenes ATCC15834 and C58C1. Hairy roots formed directly from the cut edges of leaf explants after infection for 2 days. The highest frequency of leaf explant transformation by A. rhizogenes ATCC15834 and C58C1 were 83.3\% and 80.5\% after pre culture about 2 days and infection by the bacterium containing 15 mg l-1 acetosyringone about 25 min. The PCR amplification showed that rolB genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. The optimum medium for callus induction of hairy roots consisted of 2.0 mg l-1 benzyladenine (BA) and 0.1 mg l-1 a-naphthaleneacetic acid (NAA); while optimum medium for adventitious shoot regeneration from these cultures consisted of 0.1 mg l-1 BA and 0.1 mg l-1 NAA. Adventitious shoots could be rooted on 1/2MS. PCR analysis confirmed that rol genes of TL-DNA of Ri plasmid was integrated with at least three copies into the genome of hairy roots-regenerated P. cablin plants. The results presented provide a possibility for breeding of a new cultivar of P. cablin.

}, keywords = {Agrobacterium rhizogenes, ATCC15834., C58C1, Plant regeneration, Pogostemon cablin.}, doi = {10.5530/pj.2016.1.11}, author = {Han-jing Yan and Meng-ling He and Wei-Jian Huang and Dong-mei Li and Xiao-fang Yu} } @article {44, title = {Pogostemon cablin (Blanco) Benth. (Lamiaceae): It{\textquoteright}s Ethnobotany \& in vitro regeneration}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {152-156}, type = {Original Article}, chapter = {152}, abstract = {

Objectives: Since the beginning of human civilization various herbal medicines are employed for healing human and animal. Pogostemon cablin (Blanco) Benth., locally known as Patchouli (Assamese) is a very important medicinal plants belongs to mint family i.e. Lamiaceae. The main aim of this study was to collect ethnobotanical information\’s and to study its in vitro regeneration results. All possible ethnobotanical literatures have been cited here. Methods: In vitro propagation was achieved from leaf and nodal explants of Pogostemon cablin on MS medium. Results: Callus development and in vitro axillary shoot formation was successfully made in MS basal medium containing BA (4.0 mg/L), NAA (2.0 mg/L)+ IAA (1.0 mg/L) and BA (3 mg/L)+IAA (1 mg/L). MS basal medium containing IBA (0.1/L) and Kn (2.5 mg/L) was best for induction of multiple shoots within 4 weeks of culture. Combination of NAA (0.1 mg/L). Kn (0.1 mg/L) and CH (100 mg/L) was best for callus induction which later on formed multiple shoots and caused elongation of roots. Micro shoots of varied length were produced on MS medium. Rooted plantlets were successfully acclimatized in green house for 1 month and then were transferred to the field. Conclusion: It can be concluded that pogostemon cablin has immense ethno botanical importance. For its rapid multiplication, in vitro technique was found very successful. In MS medium supplemented with Kn 2.5 mg/L and IBA 0.1mg/L found maximum multiplication rate. In this proportion rates of shoot generation, leaf, rooting, callus formation was maximum.

}, keywords = {Ethnobotany, in vitro study, MS medium, Pogostemon cablin.}, doi = {10.5530/pj.2015.3.2}, author = {Pratibha Sharma and Jintu Sarma} }