@article {1650, title = {Impact of Solvent Selection on Phytochemical Content, Recovery of Tannin and Antioxidant Activity of Quercus Infectoria Galls}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1195-1204}, type = {Research Article}, chapter = {1195}, abstract = {

Introduction: Quercus infectoria (QI) is medicinal plant well known to exhibit enormous therapeutic values. The main medicinal part of QI plant exists in its edible nutritious gall. This study aimed to determine the effects of extraction solvents on the overall phytochemical content, recovery of tannin and antioxidant activity of the QI galls. Methods: Two solvents of different polarity namely water and methanol, were used to extract bioactive compounds from the QI galls using soxhlet extraction technique. Phytochemical, TLC and HPLC assays were performed to detect and quantify the extracted compounds. DPPH assay was conducted to evaluate the antioxidant potential of the extracted compounds. Results: After 6 hours of extraction at respective solvent boiling points, methanol solvent successfully generated 1.8-fold higher crude yield (34.0\%) compared to water solvent (18.9\%). Phytochemical analysis revealed that both solvent extracts contained different secondary metabolites. The methanol extract was found richer in flavonoid, while the aqueous extract was found richer in phenol, tannin, gallotannin, triterpene and cardiac glycosides. Interestingly, we found that the major type of tannin presence in QI galls was gallotannin (hydrolysable tannin) instead of phlobatannin (non-hydrolysable tannin). The quantification results demonstrated that QI aqueous extract contained significantly higher gallotannin (75.0 μg/mL) compared to QI methanol extract (46.8 μg/mL). Furthermore, the aqueous extract also exhibited significant higher antioxidant activity compared to the methanol extract at concentration of 2 μg/mL. Conclusions: This study strongly support the utilization of water solvent for optimum gallotannin extraction from QI galls.

}, keywords = {Antioxidant, Gallotannin, Phytochemical analysis, Quercus infectoria (QI) galls, Tannin}, doi = {10.5530/pj.2021.13.153}, author = {Nur Alisa Kamarudin and Norhazilah Muhamad and Nik Nur Hakimah Nik Salleh and Suat Cheng Tan} } @article {1414, title = {Pharmacognostic Studies of the Leaves of Hyptis Suaveolens Linn. (Labiatae) (Poit)}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {May 2021}, pages = {698-705}, type = {Original Article}, chapter = {698}, abstract = {

The pharmacognostic standards of fresh, powdered and transverse sections of Hyptis suaveolens (L.) leaf were carried out to determine its macroscopic, microscopical (both qualitative and quantitative), analytical standards and phytochemical profile. The macroscopy revealed a simple leaf, oppositely arranged along the stem with a dark green colour on the outer surface and pale green on the inner surface, ovate in shape and bilateral base, acute apex with a serrate margin. The qualitative microscopy of the powdered leaf shows xylem vessels parenchymal cells with diacytic type of stomata. Also epidermal cells with stoma wall, polygonal parenchymatous and collenchymatous cells making up the cortex with starch grains; non-glandular uniserate, multicellular septate trichome, irregular shaped prisms of calcium oxalate crystals and small bundle of mucilage cells. The quantitative microscopy of the leaf showed the values of palisade ratio, stomatal number (upper and lower epidermal stomatal index (upper and lower surface), vein-islet number and vein termination number to be 5.10, 50stomata/mm2, 80 stomata/mm2, 12.33, 23.14, 35/mm2 and 20/mm2 respectively. For the analytical standards; 9.90 \%, 1.67 \%, 0.38 \% 6.39 \%, 0.61 \% w/w, 1.16 \% w/w, 11.70 \% and 7.25\% were obtained for total ash, water soluble ash, sulphated ash, acid insoluble ash, alcohol soluble extractive value, water soluble extractive value, moisture content and fibre content respectively. The qualitative phytochemical analysis on Hyptis Suaveolens leaves showed presence of carbohydrates, reducing sugar, tannins, flavonoids, steroids, glycosides, terpenoids, alkaloids and saponins. These specific standards obtained are of importance in the establishment of diagnostic indices for the standardization, identification and preparation of monograph on the plant.

}, keywords = {Analytical standards, Hyptis Suaveolens L. (Poit), Macroscopy, microscopy, Pharmacognostic standards, Phytochemical analysis}, doi = {10.5530/pj.2021.13.89}, author = {Eleje Oboma Okonta and Peculiar Feenna Onyekere and Patience Ngozi Ugwu and Helen Ogechukwu Udodeme and Vincent Obisike Chukwube and Uchenna Estella Odoh and Christopher Obodike Ezugwu} } @article {1170, title = {Pharmacognostical, Physicochemical and Phytochemical Evaluation of Strobilanthes kunthianus (Acanthaceae)}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {June 2020}, pages = {731-741}, type = {Original Article}, chapter = {731}, abstract = {

Objective: The present study aimed to evaluate pharmacognostical, physicochemical and phytochemical evaluation of various parts of Strobilanthes kunthianus. Methods: Macroscopical, microscopical, physico-chemical and phytochemical evaluations of leaves, stem, root and flowers of S. kunthianus were investigated. The paraffin embedded specimens were sectioned with the help of Rotary Microtome. The thickness of the sections was 10-12 μm and dewaxing was done by customary procedure. The sections were stained with toluidine blue for microscopical analysis. The preliminary phytochemical study was carried out for various extracts of S. kunthianus using standard procedure. Results: In the pharmacognostical, physicochemical and phytochemical studies, in an attempt to standardize the leaves, stem and root of S. kunthianus have been shown that will be definitely useful to the future researchers for the identification of the plant. Conclusion: These studies offer referential evidence for accurate identification and standardization of S. kunthianus.

}, keywords = {Pharmacognostic standardization, Physico-chemical evaluation, Phytochemical analysis, Strobilanthes kunthianus}, doi = {10.5530/pj.2020.12.106}, author = {Geetha Balasubramaniam and Mahendran Sekar and Shrishailappa Badami} } @article {840, title = {Phytochemical Analysis, In vitro Antioxidant Capacity and Toxicity Assessment of Copaifera paupera Oleoresin}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {March 2019}, pages = {374-378}, type = {Original Article}, chapter = {374}, abstract = {

Introduction: The present study was taken to determine the phytochemical analysis, the antioxidant activity in vitro (FRAP and TBARS methodologies), the oral acute toxicity and antigenotoxicity of the oleoresin Copaifera paupera. Methods: The oleoresin was tested for in vivo cytoprotective capacity using the Micronucleus Test and the Comet Assay in mice bone marrow cells and mice erythrocytes cells, respectively. Results: The oleoresin had low levels of total flavonoids and phenol content and also of antioxidant capacity. Orally, the LD50 appeared to be \> 5000 mg/kg (no toxic or low toxic). The results showed all the doses evaluated (180 360, 720 and 1440 mg/kg) antigenotoxic effect by reduce the DNA damage induced by cyclophosphamide, being a 100 \% DNA damage reduction at the highest dose evaluated. Conclusion: According to the Micronucleus test, the oleoresin Copaifera paupera had the ability of increase the cell proliferation despite the exposition of cyclophosphamide. (abstract is not arranged properly, improper sentences)

}, keywords = {Antigenotoxicity, Comet assay, Copaifera paupera, Micronucleus test, Oleoresin, Phytochemical analysis}, doi = {10.5530/pj.2019.11.57}, author = {Danae Liviac and Paola Raunelli and Rafael Alvis and Silvio Puente and Ivan Best and Oscar Reategui} } @article {822, title = {Phytochemical Compounds Of Cichorium intybus by Exploring its Antioxidant and Antidiabetic Activities}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {February 2019}, pages = {248-257}, type = {Research Article}, chapter = {248}, abstract = {

Introduction: The current study aims to evaluate the therapeutic effect of Cichorium intybus n-hexane extract on diabetes mellitus and its antioxidant effect in vivo in alloxinated animals. Diabetic neuropathy improvement was also tested as well as insulin levels and histology of the pancreas were performed. Methods: The chromatographic standardization of C. intybus extract was performed using isocratic HPLC, which indicated the presence of numerous phyto-constituents. The hexane extract was studied for its effect on blood glucose levels and painful diabetic neuropathy (DN) in diabetic mice. Hyperalgesia and mechanical-allodynia were evaluated using thermal stimuli, pain response to radiant energy experiments and a mechanical sensitivity test respectively. Subsequently, after eight weeks of being alloxinated, BGL, body weight, antioxidant activity, insulin levels and glycated hemoglobin were recorded to evaluate antidiabetic potential and the DN. Results: The administration of Cichorium intybus extract (50, 75 and 100 mg/kg) and a combination of Cichorium intybus extract and Camellia sinensis (50 + 200 mg/kg, respectively) have revealed an acute hypoglycemic effect ranging from 14.15\% and 42.4\%. The sub-chronic anti-diabetic effect ranged from 23.41\% and 44.8\%. They diminished hyperalgesia and tangible allodynia significantly (p\<0.05), (n=7 per group). The powerful neuroprotective properties might serve as potential lead-compounds for further analysis. Conclusion: The histological study and the potent antioxidant effect showed that they could assist in the management of diabetes mellitus and DN by amelioration of insulin levels and regeneration of pancreatic beta cells.

}, keywords = {Antidiabetic effects, Antioxidant, Cichorium Intybus, Phytochemical analysis, Serum insulin}, doi = {10.5530/pj.2019.11.39}, author = {Dina Kanj and Karim Raafat and Abdalla El-Lakany and Safaa Baydoun and Maha Aboul-Ela} } @article {429, title = {Chemo-profiling, Antioxidant Potential and Ionomic Analysis of Cichorium intybus L.}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {September 2017}, pages = {917-928}, type = {Original Article}, chapter = {917}, abstract = {

Background: Cichorium intybus commonly called as chicory or coffee weed was traditionally used for the treatment of various ailments associated with liver, kidney and heart. The aim of the present study was phytochemical screening, in-vitro evaluation of antioxidant potential, element analysis of wild and cultivated chicory and GC-MS analysis of methanolic leaf extract of wild chicory grown in Kashmir Himalaya. Method: To achieve this goal, the qualitative and quantitative analysis was done to determine the antioxidant potential. Element analysis was done using AAS and metabolite fingerprinting was done using GC-MS to ascertain the presence of active phytoconstituents in chicory leaves. Results: The qualitative screening of leaf extracts from wild and cultivated chicory indicates the presence of important bioactive constituents. The leaf extract of wild chicory constituted the higher amount of total phenol and flavonoid contents and also showed strong radical scavenging activities of DPPH, SOD and hydrogen peroxide radical as indicated by lower values of IC50 compared to cultivated chicory. Furthermore, both extracts of wild chicory showed the highest total antioxidant potential for the reduction of Fe3+ to Fe2+ when compared with cultivated chicory. The results from the elemental analysis shows that leaf extract of wild chicory exhibits higher concentration of elements as compared to cultivated one, thus can be used as a potential bio-fortified crop. The GC-MS analysis determines the presence of 78 different compounds in the methanolic leaf extract of wild chicory. The major phytochemical constituents were phytol (7.96\%) and stigmat-5-en-3-ol (6.45\%). Conclusion: The findings of the present study suggested the therapeutic potential of chicory leaves; particularly wild chicory proves to be a potent source of natural antioxidant against free radical associated damages. Metabolite profiling study revealed important metabolites having potent pharmacological actions.

}, keywords = {Aas, Antioxidant Activities, Cichorium Intybus, GC-MS., Phytochemical analysis}, doi = {10.5530/pj.2017.6.144}, url = {http://fulltxt.org/article/197}, author = {Bisma Malik and Tanveer Bilal Pirzadah and Inayatullah Tahir and Reiaz Ul Rehman} } @article {375, title = {Microscopical Evaluation, Phytochemical Analysis and HPTLC Fingerprinting of Tuber of Actinoscirpus grossus (L.f.) Goetgh. \& D.A.Simpson}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {July 2017}, pages = {657-662}, type = {Original Article}, chapter = {657}, abstract = {

Actinoscirpus grossus (L.f.) Goetgh. \& D.A.Simpson (Cyperaceae), is a Perennial with long stolons and rhizomes ending in small tubers. It is popularly known as Kasheruk in Sanskrit. The plant is traditionally used as anti-diarrheal, anti-emetic, and tonic to the liver. In order to do the detail standardization of plant macro-microscopical observation, phytochemical analysis and HPTLC Finger printing of tuber was performed according to pharmacopoeia procedure. Microscopic analysis has showed thick-walled polygonal epidermal cells of young root stalk in surface view, elongated phloem parenchyma filled with starch grains, spiral to annular vessel fragments and simple starch grains scattered all over the powder. Phytochemical analysis showed presence of carbohydrate, coumarins, flavanoids, steroid, tannin, and terpenoid. Ethanol extract of plant were fingerprinted in toluene: ethyl acetate (7:3). The developed plates were visualized in UV 254, 366, and then derivatised with vanillin sulphuric acid and scanned under UV 254 and 366 nm. These specific identities will be useful in identification and authentication of the raw drug.

}, keywords = {Ethanol Extract, HPTLC, Pharmacognosy, Phytochemical analysis, Quality control, standardization}, doi = {10.5530/pj.2017.5.104}, url = {/files/pj-9-5/10.5530pj.2017.5.104/index.html}, author = {Savin Chanthala Ganapathi and Rajendra Holla and Shivaraja Shankara and Sunil Kumar Koppala Narayana and Ravi Mundugaru} } @article {424, title = {Pharmacognostic Evaluation of Leaf and Stem Wood Extracts of Artocarpus hirsutus Lam.}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {September 2017}, pages = {887-894}, type = {Original Article}, chapter = {887}, abstract = {

Introduction: Artocarpus hirsutus Lam., a plant known for its fruits, used as a traditional medicine to treat skin diseases including hydrocele, pimple, heal sores, cracks in the skin. Pharmacological studies on the bark, root, leaf and fruit extracts of this species support their significance as antioxidants, as well as their efficacy on diuretic, antibacterial, anti-fungal and antiulcer activity. Methods: The present study focused on microscopic study, phytochemical analysis, HPLC and HPTLC finger print evaluation of leaf and stem wood extracts. Results: Microscopic analysis demonstrates its characteristic parameters namely hairy trichomes, pearl glands, vascular bundles, epidermis and is expected to assist easy identification of the genus. Conclusion: The solvent system, chloroform:methanol (8:2) was found to be the best as TLC eluent to visualize all major components of this species. HPLC and HPTLC finger prints not only confirm the presence of two major components, oxyresveratrol and artocarpin in stem wood extract, but also prove their absence in the leaf extract.

}, keywords = {Artocarpus hirsutus Lam., HPLC., HPTLC finger prints, microscopy, Phytochemical analysis}, doi = {10.5530/pj.2017.6.139}, url = {http://fulltxt.org/article/192}, author = {Mahadeva Nayak and Ananthanarayanan Nagarajan and Muhammed Majeed} } @article {199, title = {Physicochemical and Phytochemical Analysis of Different Parts of Indian Kesar Mango{\textendash}A unique variety from Saurashtra Region of Gujarat}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {Oct 2016}, pages = {502-506}, type = {Original Article}, chapter = {502}, abstract = {

The aim of the present study was to evaluate physicochemical and phytochemical analysis of different parts (ripe seed, unripe seed, ripe peel, unripe peel and stem) of Indian mango (var. \‘Kesar\’) collected from Saurashtra region of Gujarat. The physiochemical properties such as loss on drying, total ash value, acid insoluble ash value, water soluble ash value and extractive values were carried out. The phytochemical properties such as alkaloids, flavonoids, tannins, phlobatanins, triterpenes, steroids, saponins and cardiac glycosides were also carried out. In phytochemical analysis, tannins showed maximum amounts in all five parts. The present study provides the details physicochemical and phytochemical properties of different parts of kesar mango which are useful in laying down standardization and pharmacopeia parameters.

}, keywords = {Kesar Mango, Physicochemical parameters, Phytochemical analysis, Ripe and Unripe Peel, Ripe and Unripe Seeds, Stem.}, doi = {10.5530/pj.2016.5.16}, author = {Kalpna Rakholiya and Mital Kaneria and Sumitra Chanda} }