@article {194, title = {Analytical Quantifiation, immunomodulatory and Sulforhodamine B assay studies on Albizia lebbeck Leaves Extracts}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {Oct 2016}, pages = {476-482}, type = {Original Article}, chapter = {476}, abstract = {

Background: Albiza lebbeck leaves have been well known for its ethnopharmacological prospects. Objective: The present study aims three extracts (aqueous, methanolic and hydromethanolic) at two dose levels by oral administration by using immunomodulatory models and in vitro cell lines in correlation to analytical studies. Methods: The extracts were subjected to Haemagglutination Antibody Titre and DTH Delayed-Type Hypersensitivity reaction based on acute toxicity results. Chromatographic studies were undertaken comprising of Fourier Transform Infrared Spectroscopy and High performance Thin layer Chromatography and screened for in-vitro cell lines such as MCF-7 and HCT 15 by Sulforhodamine B Assay Method. Results: No response was shown at 100 mg/kg. Significant immunomodulatory effect was noticed at 200 mg/kg with Haemagglutination Antibody Titre (554.66 \± 102.78, 597.33 \± 85.35, 426.66 \± 53.98) and DTH Delayed- Type Hypersensitivity reaction (0.225\±0.01, 0.21 \± 0.01, 0.23 \± 0.01) which showed decrease in paw volume (after 48 h) in case of Sheep Red Blood Cells, (0.5\×109) used as antigens. Total flavonoids content in the extracts were revealed by methods described by Singleton and Quettier. Flavonols such as rutin and quercetin were detected by Fourier Transform Infrared Spectroscopy based on determination of the functional groups and High Performance Thin layer Chromatography showed well resolved spots. The extracts were screened on in-vitro cell lines (MCF 7 and HCT 15) by using Sulforhodamine B Assay method were unsatisfactory results were obtained at final concentrations of 10 \μg/ml, 20 \μg/ml, 40 \μg/ml, 80 \μg/ml. Conclusion: Thus, present paper suggests that extracts has served as a promising immunomodulator for immune system disorders.

}, keywords = {Delayed-Type Hypersensitivity response, Fourier Transform Infrared Spectroscopy, Haemagglutination Antibody Titre, High performance Thin layer Chromatography, MCF-7., Quercetin, Rutin}, doi = {10.5530/pj.2016.5.11}, author = {Gaurav Mahesh Doshi and Manjushree kundalik Pawar and Kajal Haribhai Chavda} } @article {1515, title = {Pharmacognosy and Phytochemical Analysis of Brassica juncea Seeds}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {2nd July 2014}, pages = {47-54}, type = {Original Article}, abstract = {

Introduction:Brassica juncea is an economically important plant that has been well-known in India for centuries for its medicinal and nutritive values. The broad spectrum of beneficial effects of the seeds perceived with this plant warrants further exploration of B. juncea seeds as a potential source for obtaining pharmacologically standardized phytotherapeutics, which could be potentially useful. The objective of the present study was to perform the pharmacognosy of mustards seeds inclusive of qualitative and quantitative phytochemical analysis, fingerprinting by infrared spectroscopy and high performance thin layer chromatography analysis and toxicity assessment in vitro. Methods: Different sections of seeds were taken and stained with 0.1\% phloroglucinol for microscopic examination. The seeds were extracted by 80\% alcohol on a rotary shaker to perform phytochemical analysis and fingerprinting. The toxicity assessment of this extract was performed on human dermal fibroblast cells. Results: Microscopic examination of seeds showed characteristic features of mustard seeds. The extraction of these seeds by 20\% alcohol resulted in IC50 value of 103 \± 3 \μg/mL for 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl radical scavenging assay. The fingerprinting analysis of this extract indicated probable presence of sinigrin, quercetin, vanillin, catechin, vitamin E and sulfur-containing compounds. This extract exhibited 50\% toxicity (IC50) at 1.79 mg/mL. Conclusion: The result achieved will be used to assess the therapeutic efficacy of seed extracts for future pharmacological evaluations.

Key words: Antioxidant, cytotoxicity, Fourier transform infrared spectroscopy, high performance thin layer chromatography, microscopy, phenolics.

}, keywords = {Antioxidant, Cytotoxicity, Fourier Transform Infrared Spectroscopy, High performance Thin layer Chromatography, microscopy, Phenolics}, author = {Harita Parikh and Aparna Khanna} }