@article {1656, title = {Amelioration of Cisplatin-Induced Kidney Injury by Pometia pinnata}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1257-1268}, type = {Research Article}, chapter = {1257}, abstract = {

Introduction: Cisplatin is one of the most effective anticancer drugs. But using cisplatin can cause very serious nephrotoxicity and acute kidney injury (AKI). Pometia pinnata (PE) or commonly referred to as matoa is a typical plant, especially Papua, Indonesia. Pometia pinnata belongs to the Sapindaceae family. This study aimed to determined the nephroprotective activity of the extract ethanol pometia pinnata on rats induced cisplatin. Methods: 30 rats are divided into six groups, each group were contained 5 rats. Group I was a normal group which rats only given CMC (carboxy methyl celluloce). Group II was a negative group which rats injected 7 mg / kgbw of Cisplatin in day 3. Group III was a positive group which rats given vitamin C 1\% from day 1 to 7 and in day 3 rats were injected cisplatin. Group IV-VI were extract groups (100 mg / kgbb, 200 mg / kgb, 400 mg / kgbb) which rats orally given extract from day 1 to 7 and in day 3 rats were injected cisplatin. On day 8 rats were injected ketamine 1\% which directly took the blood from the heart. Results: The result shows that EEPE on rats biochemical parameters including urea, creatinine, uric acid. Group II showed that there was a significant increase (p \<0.05) compared to the normal group that was not given cisplatin and extracts. Whereas in the group given the extract in groups IV, V, and VI there was a reduction in biochemical parameters because the Pometia leaf extract had high antioxidant activity so that it had nephroprotective activity. extract ethanol pometia pinnata can reduced the level of sodium, potassium and chloride of each group after receiving cisplatin. Statistically group II that only given cisplatin has significantly different with group I (p\<0,05) and also statically different with group VI (p\<0,05).

}, keywords = {Cisplatin, Kidney injury, Pometia pinnata}, doi = {10.5530/pj.2021.13.159}, author = {Adrian and RA Syahputra and Sukirman Lie and SE Nugraha and PC Situmorang} } @article {1156, title = {Protective Role of Ficus carica Extract Against Hepato-Testicular Side Effects and Genotoxicity Induced by Cisplatin}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {May 2020}, pages = {645-656 }, type = {Research Article}, chapter = {645}, abstract = {

Objective: The present work investigated the protective effect of Ficus carica (common fig) leaves methanol extract against genotoxicity and testicular damage of cisplatin (CP) and identified some of its active ingredients. Methods: Seven main groups were investigated as follows: I. control negative, II. Control plant (600 mg/kg fig, orally), III, IV. Control positive (treated i.p with 10 and 15 mg/kg CP), V-VII. groups treated with fig (200, 400 and 600 mg/ kg) + Cisplatin (15 mg/kg). Results: Ficus carica alleviated the destructive effects of CP in the testis, liver and bone marrow due to the presence of high amount of flavonoids and phenolic compounds. Also it has a normal effect in the tested parameters as compared with the control negative. Chromatographic investigation resulted in the identification of 6 compounds: Catechin, Luteolin-8-C-β-D glucopyranoside, Quercetin, Quercetin-3-O-β-d-glucopyranoside, Chlorogenic acid and Kaempferol-3-O-β-D-glucopyranoside. In bone marrow cisplatin induced significant percentage of chromosome abnormalities, micronuclei in polychromatic erythrocytes and toxicity to cells. On the contrary the two tested doses of cisplatin had a normal effect on spermatocyte chromosomes (germ cells). The dose 15 mg/kg induced an overexpression of the liver genes NF-kB and iNOS as indicated by real-time PCR. Different forms of histopathological alterations and instigation of the expression of TNF-α gene in the testis were detected after CP treatment. Conclusion: Ficus carica is a promising candidate rich in many bioactive constituents and can be used in combination with chemotherapeutic drugs to alleviate their destructive effects.

}, keywords = {Bone marrow, Cisplatin, Fig, Liver, Protection, Spermatocytes, Testis}, doi = {10.5530/pj.2020.12.96 }, author = {Maha A Fahmy and Entesar E Hassan and Noha E Ibrahim and Emad M Hassan and Zeinab M Hassan and Enayat A Omara} } @article {247, title = {Pharmacognostic Studies and Nephroprotective Potential of Hydroalcoholic Extract of Trichosanthes cucumerina in Acute Renal Failure}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {February 2017}, pages = {176-184}, type = {Original Article}, chapter = {176}, abstract = {

Objective: The present research work unearthed not only pharmacognostic features of the seeds of Trichosanthes cucumerina but also the nephroprotective activity of 60\% hydro alcoholic extract against Cisplatin-induced Wistar rat model. Materials and methods: Present study dealt with the detailed pharmacognostic study of the seeds of Trichosanthes cucumerina. 60\% hydro alcoholic extract was prepared by hot extraction method. Preliminary phytochemical screening was carried out. Based on acute toxicity studies nephroprotective effect of the extract was screened at 200 and 400 mg/kg, b. w. in curative and prophylactic regimen. Nephrotoxicity was induced in male Wistar rats by administration of Cisplatin (5mg/kg, b.w. i.p. as a single dose). Nephroprotective activity was assessed by estimating serum markers and urinary functional parameters supported by anti-oxidant studies and histopathological aspects. Results: Microscopic studies showed that the seed coat had outer aerenchymatous tissue, inner parenchymatous tissue and innermost compact lines of sclereids. Physicochemical evaluation yielded alcohol and water soluble extractive values of 20.8 and 8.05\%w/w. Total ash, acid insoluble and water soluble ash values were 7.15, 6.45 and 0.5 respectively. Fluorescence analysis imparted characteristic colours to the seed powder when observed under visible and UV light. Cisplatin-induced nephrotoxicity was indicated by increased levels of serum markers and urinary functional parameters which were reversed by the extract in dose dependent manner. The results were substantiated by anti-oxidant studies and histopathological studies. Conclusion: Various pharmacognostic parameters evaluated assisted in identification and standardization of seeds of Trichosanthes cucumerina in crude form. Present study revealed that hydroalcoholic extract attenuated the nephrotoxicity and provided the strengthened scientific evidence for the use of seeds of Trichosanthes cucumerina in nephrotoxicity.

}, keywords = {Anti-oxidants, Cisplatin, Histopathological studies, Pharmacognostic studies, Trichosanthes cucumerina}, doi = {10.5530/pj.2017.2.29}, url = {http://phcogj.com/fulltext/296}, author = {Doppalapudi Prasanthi and Sreedevi Adikay} } @article {159, title = {Isolation of flavonoid from Abies webbiana leaves and its activity}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {June/2016}, pages = {341-345}, type = {Original Article}, chapter = {341}, abstract = {

Background: Abies webbiana commonly known as Talispatra in Bengali and Hindi, Talispatram in Sanskrit and Indian Silver Fir in English. This is a large, tall, evergreen tree occurring in the Himalayan region from Kashmir to Assam in India. It comes under the Family: Pinaceae. The present study was designed for isolation of flavonoid from ethyl acetate extract of A. webbiana leaves and assessed their toxic effect on liver and kidney. Materials and Methods: The isolation of flavonoid using different chromatographic methods (thin layer and column chromatography). The isolated flavonoid was identified; Structures and chemical bonds were analyzed by using MP, FTIR, 1-H NMR and MS spectral analysis. Effect of flavonoid on liver and kidney was assessed by inducing (0.1 ml/kg) CCl4 (i.p.) and (6 mg/kg) Cisplatin (i.p.) respectively measured by biochemical marker of liver and kidney. Results and Discussion: It was identified that isolated compound was as 4\’-hydroxy quercetin on the basis of FTIR, 1-H NMR and MS spectral analysis. Isolated flavonoid reduced the increased biochemical marker (BM) of liver and kidney. The BM was increased by inducing CCl4 and Cisplatin respectively. Conclusion: Isolated compound was 4\’-methoxy quercetin and significantly protect the liver and kidney.

}, keywords = {1-H NMR, Abies webbiana, CCl4., Cisplatin, Quercetin}, doi = {10.5530/pj.2016.4.6}, author = {Dinesh Kumar Yadav and Mohammed Ali and Ashoke Kumar Ghosh and Babita Kumar} }