@article {2474, title = {Effects of Citrus aurantifolia Root Ethanolic Extract on Lipogenesis in Palmitate-Induced Lipid Accumulation in HepG2 Cells}, journal = {Pharmacognosy Journal}, volume = {17}, year = {2025}, month = {January 2025}, pages = {77-83}, type = {Research Article}, chapter = {77}, abstract = {

Introduction: Citrus aurantifolia (lime) is mostly found in tropical and subtropical region. The lime peel and lime juice extracts have antioxidant, antidiabetic and anti-inflammatory effects. However, the pharmacological effects of the lime root remain widely unknown. Thus, the current study investigated the effects of Citrus aurantifolia root ethanolic extract (CA) on lipogenesis induced by palmitic acid (PA) in HepG2 cells. Methods: The PA-induced lipogenesis in HepG2 cells was used for measuring lipogenic gene expression and lipid accumulation of CA. Phytochemical content was also determined in CA. Results: In PA-treated group showed the state of hepatic lipid accumulation with increased lipogenic gene, acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS) and sterol regulatory element binding protein1c (SREBP1c) as compared to the control group. Interestingly, administration of CA (5-10 μg/mL) effectively reduced lipid storage and significantly decreased the expression of these lipogenic gene in PA-treated cells. Notably, CA treatment increased the gene expression of fatty acid oxidation, carnitine palmitoyl transferase 1A (CPT1A) and peroxisome proliferator-activated receptor α (PPARα). Furthermore, this study found that the major bioactive component from CA was nordentatin (coumarin group). Conclusions: The results indicated that the CA treatment might be a useful agent for improving abnormal lipid metabolism in obesity-related nonalcoholic fatty liver disease.

}, keywords = {Citrus aurantifolia, Lipid metabolism, Lipogenesis, Nonalcoholic fatty liver disease, Obesity}, doi = {10.5530/pj.2025.17.10}, author = {Urarat Nanna and Jarinyaporn Naowaboot and Linda Chularojmontri and Rawiwun Kaewamatawong and Sudarat Homhual and Suvara Wattanapitayakul and Wanwisa Suwannaloet} } @article {2044, title = {Anti-Gastritis Activity of Cloves (Eugenia caryophyllata Thunberg) and Lime (Citrus aurantifolia) Leaf Extracts Combination in Absolute Alcohol Induced-Gastric Injury Mice}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {June 2023}, pages = {378-384}, type = {Research Article}, chapter = {378}, abstract = {

Background: Clove (Eugenia caryophyllata Thunberg) and lime (Citrus aurantifolia) are medicinal plants traditionally used to treat various diseases such as gastritis. Some studies reported that cloves leaf and lime leaf extract showed a gastroprotective effect by decreasing the gastric acid secretion and increasing the gastric mucus. Aim: This study aims to investigate the anti-gastritis activity of the combination of ethanolic extract of cloves leaf and lime leaf extract in alcohol absolute-induced mice. Methods: Mice were treated with the combination extract 0.7 g/kg BW, 1.4 g/kg BW, and 2.8 g/kg BW. Famotidine was used as a positive control, and Na CMC suspension was used as a negative control. After 45 minutes of oral administration, 0.14 ml/20g BW alcohol absolute was given to all mice. All groups were sacrificed one hour later. The evaluation showed that extract combination in all doses significantly decreased ulcer index (UI) compared to a negative control group (p\<0.005) in macroscopic evaluation. Results: In histopathologic evaluation, all doses significantly decreased mucosal edema and epithelial cell loss (p\<0.005), but in gastric bleeding evaluation, only 0.7 and 1.4 g/kg BW doses showed a significant decrease. Conclusion: This study showed that a combination of clove and lime leaf extracts has anti-gastritis activity and could be a possible therapeutic of anti-gastritis. The most effective dose is 1.4 g/kg BW in mice which showed the lowest ulcer index and gastric mucosal edema, bleeding and epithelial cells loss reduction on histopathological observation.

}, keywords = {Citrus aurantifolia, Clove, Eugenia caryophyllata, Gastritis, Lime, Ulcer}, doi = {10.5530/pj.2023.15.88}, author = {Herra Studiawan and Sukardiman and Indana Lazulfa and Rosita Handayani} }