@article {1839, title = {Protein Analysis of Royal Jelly Bee Apis Mellifera Carpatica as Candidates for Immunotherapy in Reproductive Disorders}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {338-343}, type = {Original Article }, chapter = {338}, abstract = {

Background: The honey bee of the Apis mellifera species is the main type of bee cultivated in almost all countries in the world, including Indonesia. Honey has benefits in various aspects, including food, health, and beauty. Royal jelly is a daily food menu as a food supplement to maintain and increase health and vitality. Purpose: Analyzing the immunogenic and toxinogenic proteins of Royal jelly Apis mellifera carpatica as immunotherapy. Method: Conversion of nucleotides into amino acids. Royal jelly Apis mellifera carpatica, analysis of the three-dimensional structure of Protein of Royal jelly Apis mellifera carpatica, Analysis of Ramachandran Plots of Royal jelly Apis mellifera carpatica, Analysis of epitope and allergen proteins, Analysis of antigens and toxins. Research result: The research results found that six proteins of Royal jelly Apis mellifera carpatica were epitope, antigenic, and non-toxic. Allergens and three Royal jelly proteins, Apis mellifera carpatica, are non-allergenic. In this study, the three-dimensional structure has not been found and opens opportunities for proteomic studies of the Royal jelly Apis mellifera carpatica protein, including protein isolation. Conclusion: The findings of this study can be used as a basis for the use of immunotherapy materials against the protein Royal jelly Apis mellifera carpatica against reproductive disorders.

}, keywords = {Analysis, Apis mellifera carpatica, Immunotherapy, Protein, Royal jelly}, doi = {10.5530/pj.2022.14.104}, author = {Maslichah Mafruchati and Jonathan Makuwia} } @article {1659, title = {Analysis of Three-Dimensional Protein Structure of CBAVD in Indonesia as a Basis for Immunotherapy to Ensure Maternal Health}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1282-1286}, type = {Research Article}, chapter = {1282}, abstract = {

Introduction: Congenital Bilateral Advance Vass Deferens (CBAVD) is a birth defect characterized by azoospermia. Determine the protein structure by laboratory research was relatively difficult. The threedimensional structure of proteins is computationally analyzed as an excellent and cost-effective alternative to analyzing protein characteristics. Objective: This study has an objective to identify the three-dimensional protein structure of CBAVD in Indonesia so that it can be used to obtain drugs and immunotherapy. Method: This study uses method of DNA extraction, PCR, and sequencing in collecting the data sample. The data was analyzed using using expasy software and Swiss prot. Result: The results of this study were found 6 CBAVD proteins, four to three dimensional CBAVD protein structures and 2 CBAVD proteins that have not been identified by the three dimensional protein structure. Further studies of CBAVD protein are needed, especially those related to protein isolation and crystallography. Conclusion: The three dimensional protein can be used as immunotherapy to ensure the maternal health.

}, keywords = {CBAVD, Dimensional structure, Maternal health, Protein}, doi = {10.5530/pj.2021.13.162}, author = {Maslichah Mafruchati and Jonathan Makuwira} }