@article {1958, title = {The effect of Sinensetin and Imperatorin on A-549 lung cancer cell viability in vitro}, journal = {Pharmacognosy Journal}, volume = {15}, year = {2023}, month = {March 2023}, pages = {38-46}, type = {Original Article }, chapter = {38}, abstract = {

Introduction: Lung cancer remains the leading cause of cancer death worldwide, so research is ongoing to discover new therapeutics, such as plant-derived bioactive compounds. For example, Sinensetin, a plant-derived polymethoxylated flavonoid, and Imperatorin, a natural furanocoumarin, have anti-cancer properties. This study assessed the effects of sinensetin and imperatorin separately and in combination on A-549 lung cancer cell viability. Method: The A-549 lung cancer cell line was treated with sinensetin (60 μM), imperatorin (30 M), or a combination of both compounds (Sin:Imp 30:30 μM; 50:50 μM and 60:30 μM) for 48 hours. Cell viability was then assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and apoptosis was determined using fluorescein isothiocyanate (FITC) Annexin-V/Propidium iodide staining. Results: The combination treatment of Sin:Imp 50:50 and Sin:Imp 60:30 μM reduced cell viability more than the individual treatment of sinensetin and imperatorin, with the lowest cell viability observed for the combination treatment of Sin:Imp 50:50 μM. Likewise, the combination treatment of Sin:Imp 50:50 μM induced the most apoptosis compared to individual treatment. Conclusion: Sinensetin and imperatorin can decrease A-549 lung cancer cell viability and are potent apoptotic inducers, especially when they are used in combination, therefore they are potential lung cancer therapeutics.

}, keywords = {A549, Apoptosis, Cell viability, Imperatorin, Sinensetin}, doi = {10.5530/pj.2023.15.6}, author = {Raden Anita Indriyanti and Eko Fuji Ariyanto and Hermin Aminah Usman and Ristaniah Rose Effendy and Diah Dhianawaty} } @article {1652, title = {Separation and Quantification of Sinensetin, Imperatorin and Total Tannin Content as Active Phytoconstituents of Methanol Extract of Imperata cylindrica Root from Kendari}, journal = {Pharmacognosy Journal}, volume = {13}, year = {2021}, month = {September 2021}, pages = {1216-1224}, type = {Research Article}, chapter = {1216}, abstract = {

Introduction: Imperata cylindrica root methanol extract harvested from Kendari had anti-hypertensive activity stated in a study on hypertension rat models at the doses 60 and 90 mg/kg-bw. The extract also contained phenolic compound 1.53\% (GAE) and flavonoid 1.17\% (QE) and had antioxidant activity IC50 0.32 mg/mL. Aim of the study was to determine the active secondary metabolites in the extract. Materials and Method: Sinensetin and imperatorin in the extract were separated on silica gel 60 column, eluted with a gradient eluent system as follows (1) n-hexane- ethyl acetate (3:7); (2) n-hexane {\textendash} ethyl acetate (2:8); (3) ethyl acetate -MeOH (8:2), and (4) methanol. Imperatorin and sinensetin in fractions were identified and quantified by HPLC method with column LiChroCART 250-4,6 RP 18E, isocratic mobile phases with the composition of mixture as follows, 0.1\% formic acid, acetonitrile and methanol (40:50:10) respectively, and at temperature 400 C. The total tannin was determined by spectrophotometry method with Fe(III) chloride and 1.10-Phenanthroline at wave length 510 nm. Results: Imperatorin and sinensetin were identified in fractions 5 to 9. The concentrations of sinensetin and imperatorin in Imperata cylindrica root methanol extract were of 32.348 and 37.014 mg/kg extract, respectively. The total tannin content was 1.06 \% (w/w) (TAE). Conclusion: Imperata cylindrica root methanol extract from Kendari had active phytoconstituents, namely sinensetin, imperatorin, and tannin.

}, keywords = {Imperata cylindrica, Imperatorin, Sinensetin, Tannin}, doi = {10.5530/pj.2021.13.155}, author = {Diah Dhianawaty and Mas Rizky Anggun Adipurna Syamsunarno and Resti Gradia Dwiwina and Raden Anita Indriyanti and Ruslin} }