@article {360, title = {In vitro Antibacterial Activity of Alkaloids Isolated from Leaves of Eclipta alba Against Human Pathogenic Bacteria}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {May 2017}, pages = {573-577}, type = {Original Article}, chapter = {573}, abstract = {

The susceptibility of five human pathogenic bacterial species to an alkaloids isolated from the leaves of this plant was screened using the agar well diffusion and broth micro-dilution assay. The purity of isolated alkaloids was checked by TLC and qualitative phytochemical analysis and total alkaloids were quantified. In the present study, the inhibitory action of the alkaloid was found to increase with an increase in concentration against all bacterial strains. The maximum zone of inhibition was observed at the concentration of 500 \μg/ml against all the bacteria. In this study, the S. aureus and E. coli are the more susceptible than the other selected human pathogenic bacteria. Based on the observations, E. alba appears to be a valuable source for antimicrobial properties and helps to produce antimicrobial agents to treat human pathogenic infections. Context: Different parts of Eclipta alba crude extracts are used traditionally for the treatment of several diseases of liver, skin and stomach. Aims: To study the in vitro antimicrobial activity of alkaloids isolated from E. alba against human pathogenic bacteria. Settings and Design: The susceptibility of five human pathogenic bacterial species to an alkaloids isolated from the leaves of this plant was screened using the agar well diffusion and broth micro-dilution assay. Methods and Material: The purity of isolated alkaloids was checked by TLC and qualitative phytochemical analysis and total alkaloids were quantified. Agar-well diffusion and Broth micro-dilution methods were selected to assess the antimicrobial activity. Statistical analysis used: One-way analysis of variance (ANOVA) was used to determine statistical significance. Results: In the present study, the inhibitory action of the alkaloid was found to increase with an increase in concentration against all bacterial strains. The maximum zone of inhibition was observed at the concentration of 500 \μg/ml against all the bacteria. In this study, the S. aureus and E. coli are the more susceptible than the other selected human pathogenic bacteria. Conclusion: Based on the observations, E. alba appears to be a valuable source for antimicrobial properties and helps to produce antimicrobial agents to treat human pathogenic infections.

}, keywords = {Agarwell diffusion, Alkaloid, Antimicrobial activity, Broth micro-dilution assay., Eclipta alba, Human pathogenic bacteria}, doi = {10.5530/pj.2017.4.91}, url = {/files/PJ-9-4/10.5530pj.2017.4.91}, author = {Swapna Gurrapu and Estari Mamidala} } @article {128, title = {Validated analytical RP-HPLC method for quantitation of wedelolactone from Eclipta alba and marketed Ayurvedic formulations}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {December 2015}, pages = {132-139}, type = {Original Article}, chapter = {132}, abstract = {

Introduction: Eclipta alba Linn. (Asteraceae) is an important ingredient of several Ayurvedic formulations. The monograph on different parts of plant like flowers, leaves, roots are listed in Ayurvedic pharmacopeia of India. The plant is reported to be effective for broad range maladies like inflammation, reproductive problems of females etc. It is also used as a hepatoprotectant, analgesic, antibacterial and antidiabetic agent. Wedelolactone is used as a bioactive marker to establish the quality of the crude drug and its formulations. In the present study, wedelolactone-based standardization of Eclipta alba and its quantitation from marketed herbal and Ayurvedic formulations has been documented using RP-HPLC. Methods: In the current work, an isocratic method has been developed and validated to quantitate wedelolactone from whole plant of Eclipta alba. This method is validated as per ICH guidelines and is used to quantitate the content of wedelolactone in polyherbal formulations like Liv52, Geriforte, Mahabhringaraj oil etc. Results: The LOD is found to be 0.5 \μg/mL and the LOQ is 1 \μg/mL. The linearity range of response is from 5 \μg/mL to 100 \μg/mL. Conclusion: The validated method is found to be simple, sensitive, accurate, rugged and reproducible. This developed method can be recommended for quality assurance and marker-based standardization of polyherbal formulations containing whole plant of Eclipta alba.

}, keywords = {Eclipta alba, Formulations, RP-HPLC, Validation, Wedelolactone., Whole plant}, doi = {10.5530/pj.2016.2.6}, author = {Sunita Shailajan and Sasikumar Menon and Dipti Singh and Gauri Swar} }