@article {896, title = {Screening and Evaluation of Lectin and Anti-Cancer Activity from the Phloem Exudate/Sap of the Indian Dietary Ethnomedicinal Plants}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {May 2019}, pages = {570-578}, type = {Original Article}, chapter = {570}, abstract = {

Objective: Lectins are extremely significant biomolecules to study several biological progressions. In this present investigation, we are screening the crude phloem exudate/ sap sample from different ethnomedicinal plants were evaluated for lectin and anticancer activity. Methods: The lectin activity of crude phloem exudate/sap samples were confirmed by haemgglutination assay and anticancer activity by using trypan blue, MTT and in-ovo CAM angiogenic assay. The tumor cell nuclei resulting in Giemsa stain, AO/EtBr stain, DNA Fragmentation and Caspase- 3 inhibitor assay. Results: Our experimental data show that the phloem exudate/sap sample S2 (Musa Acuminata), sample S4 (Euphorbia Geniculate) exerting the potent lectin activity, sample S5 exerting very low lectin activity against the trypsinized rabbit erythrocytes and decreases the cell viability in EAC cells in-vitro. Sample S2, S4 and S5 exerts significant cytotoxic effect against the various human cancer cell lines and regressed the neovasculature (development of new blood vessels) in the developing CAM embryos when compared to the other crude samples. The apoptotic inducing activity of crude phloem exudate/sap samples was revealed by DNA fragmentation assay, caspase-3 inhibitor assay and cellular morphology were studied by fluorescence staining methods. Conclusion: This study reports that some of the isolated crude phloem exudate/sap samples show potent lectin activity and anti-cancer activity in different human cancer cell lines. The further additional experiment needs to purify and characterize the bioactive lectin components from the potent sample which is responsible for pro-apoptotic, anti-angiogenic activity and mechanism involved.

}, keywords = {Angiogenesis, Apoptosis, EAC, Haemagglutination, Lectin, VEGF}, doi = {10.5530/pj.2019.11.91}, author = {Balaji Kyathegowdanadoddi Srinivas and Madhu Chakkere Shivamadhu and Preethi Saligrama Devegowda and Gurukar Mathew and Theethagounder Tamizhmani and Senthilkumar Gnanavadevel Prabhakaran and Shankar Jayarama} } @article {113, title = {Role of Plant Based Lectins in Identifying Rare Bombay Blood Group}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {09/2015}, pages = {70-71}, type = {Original Article}, chapter = {70}, abstract = {

Background: Lectins are proteins found in a diversity of plants and organisms. They possess the ability to agglutinate erythrocytes with known carbohydrate specificity. Methods: A lectin named Anti H is used in identification of a very rare and important blood group named Bombay blood group is extracted from the plant Ulex europaeus. Our study was done to calculate the prevalence of Bombay blood group in our Indian population using the plant based extract lectin anti H. Blood grouping was done using the tube technique using commercially available antisera. Results: Out of the total 11,512 blood samples collected and analysed, two samples were identified to be Bombay blood group. The prevalence of Bombay blood group in our study is .017\%. The prevalence of the Bombay blood group varied slightly among different regions in parts of India. Conclusion: Anti H Lectin helped in identifying Bombay Blood Group which would otherwise have been missed in routine blood grouping. Plant based lectins will perform an important role in the field of transfusion medicine in near future.

}, keywords = {Blood grouping, Bombay phenotype, H antigen, Lectin, Ulex Europaeus.}, doi = {10.5530/pj.2016.1.15}, author = {Raj Bharath Rudrappan and Krishnan Veeran} }