@article {2436, title = {Natural Cartilage-Derived Scaffolds for 3D Mesenchymal Stem Cell Culture: Promoting Chondrogenesis and Modulating Secretome Composition}, journal = {Pharmacognosy Journal}, volume = {16}, year = {2024}, month = {December 2024}, pages = {1365-1372}, type = {Research Article}, chapter = {1365}, abstract = {

Introduction: The regenerative potential of stem cells lies in their secretome, a collection of signaling molecules whose composition is shaped by the cellular microenvironment and external stimuli. To enhance cartilage regeneration, a 3D culture system using a natural cartilage scaffold has been developed to create a more chondrogenic secretome. This study investigates the chondrogenic differentiation of mesenchymal stem cells (MSCs) cultured in a decellularized bovine cartilage scaffold and analyzes the resulting secretome{\textquoteright}s composition. Methods: This study employed a randomized time series design to examine MSCs chondrogenic differentiation. A control group was cultured in standard 2D conditions, while two experimental groups were cultured in either 2D medium supplemented with chondrocyte differentiation medium (positive control) or a 3D decellularized bovine cartilage scaffold. The study hypothesized that the 3D culture would promote chondrogenesis at least as effectively as the positive control. Key chondrogenic markers were evaluated at various time points. Results: Statistical analysis revealed significant differences in marker expression between the experimental and control groups. SOX-9 and aggrecan were elevated in both experimental groups. The 3D group showed higher RUNX-2 expression and the highest Coll-2 expression at later time points. Additionally, growth factor analysis showed the 3D group had the highest levels of IGF-1 and FGF-2 towards the end of the study. Conclusion: 3D culture of MSCs in a bovine cartilage scaffold enhances chondrogenic differentiation and produces a secretome with comparable chondrogenic potential to traditional 2D culture with differentiation medium, suggesting its promise for cartilage regeneration.

}, keywords = {Cartilage, Culture Technique, Secretome, Stem Cells, Three-Dimensional}, doi = {10.5530/pj.2024.16.220}, author = {Kukuh Dwiputra Hernugrahanto and Naufaldy Rifqiaulia Noerda and Jifaldi Afrian Maharaja Dinda Sedar and Lukas Widhiyanto and Dwikora Novembri Utomo and Djoko Santoso} } @article {1178, title = {Human Umbilical Cord Blood-derived Secretome Enhance Endothelial Progenitor Cells Migration on Hyperglycemic Conditions}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {June 2020}, pages = {793-797}, type = {Original Article}, chapter = {793}, abstract = {

Hyperglycemia state is harmful to body{\textquoteright}s homeostasis. Uncontrolled hyperglycemic patients, especially patients with diabetes mellitus have a higher mortality risk of heart disease 2 to 4 times compared to non-hyperglycemic patients. Vascular endothelial impairment always been observed and found as a key feature of hyperglycemia state, which is correlated with reduced numbers and dysfunction of endothelial progenitor cells (EPCs). Objective: This paper aims to investigate the effect of hUCB-MSCs derived secretome treatment on the EPCs migration under hyperglycemia state. Materials and Methods: EPCs were isolated and cultured from peripheral blood samples and cultured for three days. Cultured EPCs were cultivated in 6-well plates until confluence and incubated with high glucose for 5 days, then placed in the modified Boyden chamber at the upper chamber with basal media. The lower chamber was supplemented with basal media and secretome at 2\%, 10\%, and 20\% concentration and VEGF treated group as a control. EPCS migration was evaluated using a Boyden chamber assay. Statistical analysis was performed using SPS 25.0. Results: EPCs migration were significantly higher when hUCB-MSCs-derived secretome was given in high glucose concentrations compared to the and control group (79.80 {\textpm} 5.07 vs 51.00 {\textpm} 5.15, p\<0.000). This study also showed that hUCB-MSCs-derived secretome increase EPCs migration under high glucose concentrations in a dose-dependent manner (p\<0.05). Conclusion: hUCB-MSCsderived secretome enhances EPCs migration under hyperglycemic state. This result may be of relevance for cell-free and regenerative therapeutic modality for a diabetic patient with coronary artery disease (CAD).

}, keywords = {Endothelial progenitor cells, Hyperglycemia state, Mesenchymal stem cells, Migration, Secretome}, doi = {10.5530/pj.2020.12.113}, author = {Yudi Her Oktaviono and Melly Susanti and Achmad Lefi and Ferry Sandra} }