@article {108, title = {Estrogenic Potential of Flemingia vestita Benth Tubers in Ovariectomized Rat Model}, journal = {Pharmacognosy Journal}, volume = {8}, year = {2016}, month = {09/2015}, pages = {44-49}, type = {Original Article}, chapter = {44}, abstract = {

Objective: This study investigates the potential estrogenic activity of the ethanolic extract of Flemingia vestita Benth tubers using ovariectomized rat model. Materials and Methods: The ethanolic extract of F. vestita tubers has been standardized using validated HPLC method in terms of its genistein content (8.43 \± 0.05 mg/g of extract). Three to four week old young albino Wistar female rats were ovariectomized and treated for 14 days post ovariectomy with the standardized ethanolic extract at three different dose levels (100, 250, 500 mg/kg body weight) with a positive control of Estradiol valerate (1 mg/kg/day). The parameters evaluated were uterine weight, uterine glycogen, G6PDH, LDH, 17\β-estradiol, progesterone, total cholesterol, triglycerides, HDL and histo architecture of uterus. Results: Treatment with the ethanolic extract of F. vestita tubers showed dose dependent increase in uterine weight, glycogen levels, G6PDH levels, estrogen and progesterone levels when compared with the ovariectomized control. Amongst three dose levels, high dose of plant extract showed significant increase in the uterine weight (p \< 0.001), uterine glycogen content (p \< 0.001), 17-\β estradiol and progesterone levels (p \< 0.001), G6PDH and LDH levels (p \< 0.001) as well as significant decrease in HDL and triglycerides levels (p \< 0.001) compared to ovariectomized control. Histopathological evaluation of uteri sections revealed that the high dose of the plant show increase in the endometrial response as indicated by proliferation of endometrial glands and luminal epithelium of the ovariectomized rats. Conclusion: Thus, these data suggests that ethanolic extract (500 mg/kg body weight) of F. vestita tubers may exhibit good estrogenic activity in ovariectomized rat model.

Key words: Estrogenic activity, Flemingia vestita, genistein, HPLC, ovariectomized rats.

}, keywords = {Estrogenic activity, Flemingia vestita, genistein, HPLC, ovariectomized rats.}, doi = {10.5530/pj.2016.1.10}, author = {Sunita Shailajan and Suman Kumaria and Suhas Pednekar and Sasikumar Menon and Hiranjit Choudhury and Archana Matani} } @article {1502, title = {Estrogenic Activity of Bauhinia racemosa extract in Female albino rats: An Investigational Study}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {3rd Sept, 2014}, pages = {5-9}, type = {Original Article}, chapter = {5}, abstract = {

Objective: To evaluate the effect of petroleum ether, ethanol and water extract of Bauhinia racemosa in female albino rats. Methods: Plant extracts were tested for their estrogenic activity by using ovariectomised method at two dose level: 200 mg/kg and 400mg/kg respectively. Further, Plant extracts and Standard drug Ethinyl estradiol (1\µg/kg) combination were tested for synergistic estrogenic activity. Results:In-vivo investigation revealed that ethanol extract at dose of 200 mg/kg and 400 mg/kg shows significance increase 325.23\±5.23, 328.84\±4.56 and 3.252\±0.47, 3.288\±0.27 in uterine wet weight and uterine weight ratio respectively in female albino rat as compared to control and petroleum ether and water extracts. Simultaneous administration of standard drug Ethinyl estradiol (1\µg/kg) with ethanol extract at dose of 200 mg/kg and 400 mg/ kg potentiates (327.36 \± 5.78, 330.95 \± 6.21 and 3.273 \± 0.64, 3.309 \± 0.49 uterine wet weight and uterine weight ratio respectively in female albino rat) the estrogenic activity as compared to individual administration of ethanol extract as a synergistic effect. Conclusion: It was observed that ethanol extract of Bauhinia racemosa produced significance estrogenic activity.

Key words:Bauhinia racemosa, estrogenic activity, Ethinyl estradiol, ovariectomy.

}, keywords = {Bauhinia racemosa, Estrogenic activity, Ethinyl estradiol, ovariectomy.}, author = {Gautam P. Vadnere and Aslam R. Pathan and Abhay K. Singhai} } @article {1519, title = {Estrogenic Effect of Methanolic Extract of Avicennia alba Blume. Aerial Parts in Female Wistar Albino Rats}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {26th May 2014}, pages = {53-58}, type = {Research Article}, abstract = {

Objectives: To investigate the estrogenic effect of Avicennia alba (Blume) aerial parts as identified in the folklore recipes as a contraceptive Methodology: The estrogenic activity study of the methanolic extract was carried out by studying the histopathology of the uterus. The uterine weight and vaginal cornification were also observed. The diameter of uterus, thickness of endometrium and height of the endometrial epithelium were measured using a calibrated ocular micrometer. The estimation of total protein and cholesterol was carried out using a standard method described by Lowely et al. Results: The presence of alkaloids, anthraquinone glycosides, flavonoids, steroids, polyphenolics were detected in the aerial parts of Avicennia alba. The methanolic extract of Avicennia alba at 400 mg/kg body weight showed a significant (p\< 0.05) increase in uterine weight, diameter of uterus, thickness of endometrium and height of the endometrial epithelium compared to the control. There was increase in vaginal cornification status. The histological examination of the uterus also showed estrogenic influence. A significant increase (p\< 0.05) in total protein and cholesterol content in the uterus of standard and test drug treated rats was also observed. Conclusion: The methanolic extract of Avicennia alba at 400 mg/kg body weight showed significant estrogenic activity and the results are in consistent with the folkloric claim reports related to oral contraceptive effect of Avicennia alba

Key words:Avicennia alba, contraceptive, estrogenic activity, uterus.

}, keywords = {Avicennia alba, contraceptive, Estrogenic activity, Uterus}, author = {D R Kar and G Ghosh and P K Sahu} }