@article {2246,
	title = {Boletus griseipurpureus Corner: Antibacterial, Antioxidant Properties and Phytochemical Compositions},
	journal = {Pharmacognosy Journal},
	volume = {16},
	year = {2024},
	month = {April 2024},
	pages = {296-301},
	type = {Original Article},
	chapter = {296},
	abstract = {<p class="rtejustify">This study aims to investigate the properties of B. griseipurpureus Corner concerning its efficacy against various bacterial strains and its antioxidant capacity, along with its phytochemical composition. The antibacterial activity was evaluated using the agar well diffusion method, revealing pronounced efficacy against gram-negative bacteria, particularly noteworthy against E. coli ESBL182, known for its antibiotic resistance. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of B. griseipurpureus Corner extract against E. coli ESBL182 were determined to be 75 and 300 mg/ml, respectively. The extract exhibited bacteriostatic activity by inhibiting cell growth, leading to a reduction in optical density at 600 nm. Additionally, examination of the supernatant from untreated and treated E. coli ESBL182 cells with B. griseipurpureus Corner extract indicated absorbance values of 0.088 and 0.248 at 260 nm, and 0.045 and 0.286 at 280 nm, respectively. Further investigation utilizing a scanning electron microscope revealed alterations in the morphology of treated cells, which displayed elongation and fragmentation, in contrast to untreated cells. The DPPH assay indicated that the IC50 of B. griseipurpureus Corner extract was 31.22 mg/ml. The IC50 value obtained from the ABTS assay was 47.31 mg/ml. Additionally, the FRAP assay revealed that the concentration of ascorbic acid equivalent in B. griseipurpureus Corner extract was 1.06 mg/g crude extract. Phytochemical analysis, conducted using a spectrophotometer at wavelengths of 750 nm and 510 nm, respectively, indicated phenolic and flavonoid contents of 0.22 mg gallic acid/g fresh weight and 3.23 mg quercetin/g fresh weight in the extract.</p>
},
	keywords = {Antibacterial activity, Antioxidant property, Boletus griseipurpureus Corner, Flavonoid compound, Phenolic compound, Phytochemical compositions},
	doi = {10.5530/pj.2024.16.44},
	author = {On-Anong Somsap and Anussara Kamnate and Amornrat Angajchariya}
}
@article {960,
	title = {Evaluation of Antioxidant Activity and Phytochemical Screening of Leaves, Barks, Stems and Fruits of Alphitonia philippinensis (Rhamnaceae) From Brunei Darussalam},
	journal = {Pharmacognosy Journal},
	volume = {11},
	year = {2019},
	month = {September 2019},
	pages = {951-961},
	type = {Original Article},
	chapter = {951},
	abstract = {<p class="rtejustify">Phytochemical screening of Alphitonia philippinensis extracts revealed the presence of cardiac glycosides, tannins, saponins and steroids in different plant parts. Total phenolic content (TPC), total flavonoid content (TFC) and total flavonol content (TFlC) were measured using spectrophotometric methods where gallic acid and quercetin were the standards. Antioxidant activity of extracts was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2̉-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS), free radical scavenging and ferric reducing antioxidant power (FRAP) assays using ascorbic acid and trolox were used as standards. Among the four different crude methanol extracts studies, leaves showed highest antioxidant capacity. EC<sub>50</sub> values of the leaves extract from DPPH and ABTS method found 32 and 45 μg/mL respectively. The higher antioxidant activity of the leaves extract could be correlated with the presence of higher total phenolic content, total flavonoid, and total flavonol contents. According to antioxidant contents and assay results leaves extract possesses highest antioxidant property following fruits, barks and stems.</p>
},
	keywords = {Antioxidant property, philippinensis, Phytochemical investigation, Total Phenolic Contents},
	doi = {10.5530/pj.2019.11.151},
	author = {Jamiuddin Ahmed and Kamariah Abu Salim and Linda B.L. Lim and Abdalla Mohamed Jama}
}
