@article {945, title = {Glucosinolates, Degradation Products and Myrosinase Activity in Raphanus sativus Linn.}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {September 2019}, pages = {866-872}, type = {Original Article}, chapter = {866}, abstract = {

This research was conducted to assess the gluocosinolate (GSL), isothiocyanate (ITC) and myrosinase content in two cultivars of Raphanus sativus Linn. (white and red) roots. LC-ESIMS investigation was done on desulfated GSLs since this methodology has been previously established for efficient GSL analyses. The major GSLs: sinigrin (1) 1,2-dihydroxy-2-phenylethyl glucosinolate (2), 4-hydroxyglucobrassicin (3), glucoraphasatin (4) and 4-methoxyglucobrassicin (5) were found in red radishes; whereas, only 1 and 4 were obtained in white radishes. Myrosinase was analyzed in the tubers due to its ability to catalyze and hydrolyze GSLs into ITCs. This β-thioglucosidase enzyme was found to be over 10 times more active in red tubers (2.05E-02 units) than in white radishes (1.55E-03 units) and the results were linked to the presence/absence of the outer covering of the tubers. Due to the promising medicinal properties of the aglucone derivative of compound 4, 4-methylthio-3-butenyl isothiocyanate (6), the ITC analog was monitored using gas chromatographic mass spectral analyses after myrosinase-mediated hydrolysis. From the results, it can be construed that the occurrence of GSLs 1-5 and the bioactive agent 6 were inherent in the R. sativus cultivars evaluated.

}, keywords = {GC-FID, GC-MS, Glucosinolates, Isothiocyanates, LC-ESI-MS, Myrosinase}, doi = {10.5530/pj.2019.11.139}, author = {Maria Carmen S. Tan and Marissa G. Noel} }