@article {1842, title = {Amino Acid and Proximate Analysis of Type-1 Collagen from Sea Cucumber and Tilapia-Skin and its Potential Application as Artificial Tendon}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {358-361}, type = {Original Article }, chapter = {358}, abstract = {

Tendon injury is a medical indication that requires surgical therapy and prolonged recovery. Collagen plays a crucial role in the wound healing process. Collagen maintains homeostasis, interacts with platelets, and enhances cellular components. Most living organisms consist of collagen components as fibrillar proteins. In this study, the potential properties of type-1 collagen from sea cucumber and Tilapia-skin were evaluated depend on proximate analysis, amino acid composition, and moisture-absorption ability. The result showed that Tilapia-skin has a higher protein and fat composition compared to the sea cucumber. In contrast, moisture, ash, and yield composition showed higher for sea cucumber compared to Tilapiaskin. Meanwhile, based on amino acid analysis was revealed that proline, alanine, methionine, isoleucine, leucine, phenylalanine, and lysine were lower on sea cucumber compared to Tilapia-skin. In conclusion, both type-1 collagen material from sea cucumber and Tilapia-skin might be developed as a biomaterial for tendon injury. It is crucial that further studies be carried out to emphasize the potential of biodegradable and bio-replaceable materials using the in vivo model.

}, keywords = {collagen, Key words: Artificial tendon, Marine biodiversity, Sea cucumber, Tilapia-skin}, doi = {10.5530/pj.2022.14.107}, author = {Arifia Safira and Cinta Atsa Mahesa Rani and Roro Ayu Puspitasari and Anindyta Kirana Putri Ayuningtyas and Yayang Amru Mahendra and Agus Purnomo and Faisal Fikri and Shekhar Chhetri and Muhammad Thohawi Elziyad Purnama} } @article {918, title = {Screening of Tyrosinase Inhibitor, Antioxidant and Cytotoxicity of Dried Sea Cucumber from Tomini Bay, Indonesia}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {May 2019}, pages = {555-558}, type = {Original Article}, chapter = {555}, abstract = {

Background: Indonesia, as a tropical country, is one of the important producers of sea cucumbers (beche-de-mer). Sea cucumber is a marine invertebrate that contains attractive bioactive secondary metabolites and these metabolites can be used for health as well as cosmetics. Objective: The aim of the study was to determine the activity of tyrosinase inhibitors, antioxidants, and cytotoxicity of sea cucumber methanolic extract. Methods: Dried sea cucumber samples were taken from Boalemo waters, Tomini Bay, Indonesia. Tyrosinase inhibitor assay was carried out spectrophotometrically using tyrosinase enzymes and L-DOPA as a substrate and antioxidant tests were carried out by DPPH method. Cytotoxicity test against human breast cancer cell line (T47D) was conducted using the MTT assay. Results: The study showed that Bohadschia vitiensis had the best tyrosinase inhibitor activity with IC50 value of 0.28 mg/ml. The DPPH free radical scavenging testing showed that all sea cucumbers had weak antioxidant activity. On the other hand, cytotoxicity assay revealed that several sea cucumbers had good cytotoxicity against T47D cells, where Holothuria atra and Bohadschia marmorata showed strong cytotoxicities with IC50 values of 23.0 and 28.1 ug/mL, respectively. Conclusion: Based on the study, it can be concluded that the dried sea cucumber from the Tomini bay region, Indonesia, has the potential to be developed as a source of tyrosinase inhibitors and cytotoxic agents.

}, keywords = {Antioxidant, Cytotoxicity, Screening, Sea cucumber, Tyrosinase inhibitor}, doi = {10.5530/pj.2019.11.88}, author = {Muhammad Nursid and Endar Marraskuranto and Azizah Kuswardini and Tjahyo Winanto} }