@article {1023, title = {Inhibitory Effects of Sangketan (Achyranthes aspera L.) Roots Extract on Arginase Activity and Determination of Its Total Phenolic and Flavonoid Contents}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {October 2019}, pages = {1231-1234}, type = {Original Article}, chapter = {1231}, abstract = {

Background: Achyranthes aspera, or commonly called as Sangketan in Indonesian is a wild plant that is used as a traditional medicine. The roots of Sangketan can be used as a wound healer by involving the role of arginine and its metabolites, nitric oxide, that directly affect the wound healing process itself. Objective: The aim of this study is to determine the potential of Sangketan roots extract in inhibiting arginase activity. Methods: The roots were extracted using multistage ultrasound-assisted extraction method with n-hexane, ethyl acetate and methanol solvent. Each extract from different solvents was tested for the inhibition of arginase activity using a microplate-based colorimetric method, followed by determination of total phenolic concentration and total flavonoid concentration. Results: The results of inhibition test of arginase activity by n-hexane, ethyl acetate and methanolic extracts were 9.56; 17.58; and 29.77\% sequentially/respectively at concentration of 100 μg/ml; the total phenolic concentration were 3.91; 4.83; dan 11.18 mgGAE/g of sample respectively; and the total flavonoid concentration are 0.29; 0.80; and 0.88 mgQE/g of sample respectively. Conclusion: From this research, it can be concluded that Sangketan roots extract had low potency of arginase inhibitory activity.

}, keywords = {Achyranthes aspera, Arginase, Inhibitory effect, Sangketan, total flavonoid content, total phenolic content}, doi = {10.5530/pj.2019.11.191}, author = {Dieah Siti Rahmawati and Berna Elya and Arikadia Noviani} } @article {86, title = {Preliminary Phytochemical Analysis and In vitro Anti-helmenthic activity of Achyranthes aspera Leaf extract}, journal = {Pharmacognosy Journal}, volume = {7}, year = {2015}, month = {01/2015}, pages = {397-399}, type = {Original Article}, chapter = {397}, abstract = {

Objective: To evaluate the preliminary phytochemical analysis and in vitro- Antihelmenthic activity of leaf extract of Achyranthes aspera. Background: Achyranthes aspera is an annual, stiff-erect herb found commonly as a weed throughout India. The leaf ethyl acetate extract showed high larvicidal activity on the tick larvae of Rhipicephalus. It strengthen the liver and kidneys, strengthening muscles, tendons and bones, anti-inflammatory, anti-toxin, urine laxative, normalize menstruation, hemostatic, ease childbirth. Chemical Ingredients includes Akirantin, glokosa, galactose, reilosa, ramnosa, and alkaloids. Hentriakontan, sapogenin, Betaine, ecdysterone, triterpenoid saponins. Methods: Preliminary phytochemical analysis was done by adopting the method of Evans. Antihelmenthic assay is performed by standard protocol. Result: The present study reveals the presence of few secondary metabolites and the extract exhibitspotent Antihelmenthic activity. Conclusion: From the present study it can be concluded that Achyranthes aspera leaf extract possessed marked in vitro Anti helminthic effect.

}, keywords = {Achyranthes aspera, Anti-helmenthic, Anti-inflammatory, Laxative, Phytochemical.}, doi = {10.5530/pj.2015.6.14}, author = {Kamal Hasan and Thangavelu Lakshmi and Thirumalai Kumaran Rathinam} }