@article {1221, title = {Chemical Composition and Content of Polysaccharides from the Yellow Iris (Iris pseudacorus L.) Rhizomes}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {August 2020}, pages = {1012-1018}, type = {Original Article}, chapter = {1012}, abstract = {

Background: The aim of the present study was to examine the composition and content of the polysaccharide complex and polysaccharide fractions from yellow iris (Iris pseudacorus L.) rhizomes growing in Russian Federation (Moscow region). Materials and Methods: Traditional pharmacognostic methods were used in the analysis of polysaccharides as biologically active substances. The total amount of polysaccharides was determined by a gravimetric procedure with alcohol precipitation. Fractions of polysaccharides were isolated by sequential changing of solvents. The content of monosaccharides was determined by the method of high-performance liquid chromatography with refractometric detection (HPLC-RID) after acid hydrolysis. HPLCRID procedure was performed using Chromatograph Agilent 1260 Infinity, Sugar-Pak column (300{\texttimes}6.5 mm), and isocratic elution mode. Results: Polysaccharide complex was isolated, the total content of polysaccharides in the rhizomes of I. pseudacorus was determined, as well as their distribution by fractions (water-soluble polysaccharides, pectic substances, hemicellulose A, hemicellulose B), the composition of individual sugars (glucose, xylose, galactose, mannose, rhamnose, arabinose) was determined for each fraction. Conclusion: In our experiment polysaccharide complex was first identified and described in detail for I. pseudacorus grown in Moscow region.

}, keywords = {Hemicellulose, Iris pseudacorus L., Pectins, Polysaccharides, Water-soluble Polysaccharides, Yellow iris}, doi = {10.5530/pj.2020.12.143}, author = {Tikhomirova EA and Sorokina AA and Bubenchikova VN and Kostikova EN and Zhilkina VYu and Bessonov VV} } @article {878, title = {In vivo Toxicopathological Evaluation of a Purified Bioflocculant Produced by Arthrobacter humicola}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {May 2019}, pages = {486-492}, type = {Original Article}, chapter = {486}, abstract = {

Introduction: In spite of the commendable flocculating activities of microbial flocculants, a good number of them potentiate significant toxicity. This study evaluated the in vivo toxicological implications of treatment with the Purified bioflocculant (PB) from Arthrobacter humicola using OECD guidelines. Materials and Methods: In the acute toxicity assessment, a single oral administration of 2000 mg/kg body weight (b.w.) of PB was given to the Wistar rats and the animals were observed for 2 weeks. The daily dose toxicity testing was performed through daily oral treatment with graded doses (50, 200 and 500 mg/kg b.w.) of PB for 4 weeks. Clinical signs of toxicity, behavioral changes, hematological and biochemical parameters were thereafter evaluated. Results: PB at 2000 mg/kg b.w. produced no treatment-mediated signs of toxicity, behavioral changes or mortality in the animals. Thus, its no-observed-adverse-effect level was estimated to be above 2000 mg/kg b.w. In the repeated dose toxicity testing, treatments with PB also revealed no significant differences in the feeding patterns, lipid profiles, hematological and clinical biochemistry parameters when compared with the control group. Although, at 500 mg/kg b.w. PB, a significant increase was observed in the serum activities of alkaline phosphatase, nonetheless, cage side observations recorded no treatment-induced signs of toxicity and macro-histopathological examinations of all the investigated organs also revealed no obvious morphological changes. Conclusion: The overall results suggested that PB was well tolerated by the animals and is endowed with monosaccharides bearing functional groups of flocculation importance, thus, suggesting its potential application as a safe actinomycetes bioflocculant for water treatment.\ 

}, keywords = {Actinomycetes, Arthrobacter humicola, Bioflocculant, Hematological, Polysaccharides, Wastewater treatment}, doi = {10.5530/pj.2019.11.77}, author = {Mayowa Oladele Agunbiade and Sabiu Saheed and Esta Van Heerden and Carolina Henritta Pohl} } @article {889, title = {Phytochemical Study of Odorous Celery Root (Apium graveolens L.) Grown in the North Caucasus}, journal = {Pharmacognosy Journal}, volume = {11}, year = {2019}, month = {May 2019}, pages = {527-530}, type = {Original Article}, chapter = {527}, abstract = {

Context: Flavoring celery (Apium graveolens L.) from the umbrella family (Apiaceae) is a common food plant, its use as a spice has been known since Ancient Greece. Stems, leaves and root are widely used in cooking in various dishes: soups, salads and side dishes. Celery fragrant has mild laxative, diuretic, antiseptic, anti-allergic, anti-inflammatory, enveloping and wound healing properties. Aims: The aim of our research was the phytochemical study of celery root odorous, quantitative definition of flavonoid, carotenoid, polysaccharides, tannin, free organic acids, essential oils and lipid. Methods and Material: The research material was dried and chopped celery fragrant root passing through a 3 mm sieve. We carried out quantitative determination of biologically active substances in the celery root odorous by the following methods: flavonoids (In terms of rutin) by the spectrophotometric method; carotenoids by the photocolorimetric method; essential oil, polysaccharides, lipophilic substances by the gravimetric method; free organic acids and tannins by the titrimetric method. Statistical analysis used: The results of the studies are given in the table. Statistical data processing was performed according to the method {\textquotedblleft}Statistical processing of the results of a chemical experiment and biological tests{\textquotedblright} presented in State Pharmacopoeia XI [1]. The sample size (n) is 6, the confidence level (P) is 95\%, the student criterion (t (P, f) is 2.57). Results: For the first time, we carried out a quantitative assessment of the content of seven groups of biologically active compounds of celery root odorous, grown in the North Caucasus: essential oil - 2.04 {\textpm} 0.01\%, flavonoids - 1.44 {\textpm} 0.01,\% lipophilic substances - 4, 05 {\textpm} 0.01\%, carotenoids - 3.03 {\textpm} 0.02\%, free organic acids - 2.38 {\textpm} 0.02\%, tannins - 8.06 {\textpm} 0.01\%, water-soluble polysaccharides - 8, 33 {\textpm} 0.01\%, pectin substances - 3.45 {\textpm} 0.01\%, hemicellulose A - 1.54 {\textpm} 0.01\%, hemicellulose B - 1.47 {\textpm} 0.01\%. Conclusion: Celery root odorous grown in the North Caucasus is a source of biologically active compounds.

}, keywords = {Carotenoids, Essential oil, Flavonoids, Free organic acids, Polysaccharides, Tannins}, doi = {10.5530/pj.2019.11.84}, author = {Tsakhkhaeva Zuhra Sarmanovna} } @article {1512, title = {Immunomodulatory Effect of Water Soluble Polysaccharides Isolated from Metroxylon sagu in Animal Models of Immunosuppression}, journal = {Pharmacognosy Journal}, volume = {6}, year = {2014}, month = {2nd July 2014}, pages = {55-62}, type = {Original Article}, abstract = {

Aim: This study was aimed to investigate the immunomodulatory activity of water soluble polysaccharides isolated from Metroxylon sagu (PSMS) by dilute acid extraction, ethanol precipitation in rats by using three different in-vivo experimental models of immunosuppression. Methodology: Three models of immunosuppression include metronidazole (MTZ) induced immunosuppression, pyrogallol induced immunosuppression and Ethanol-induced immunosuppression. Immunological indices like humoral antibody titer values, cellular immune response, percent change in phagocytosis, serum immunoglobulins were estimated. Histopathology of spleen was done in all control and treated groups. The doses of 500 and 250 mg/kg of PSMS were administered orally to evaluate the immunomodulatory activity. Results: Though PSMS was demonstrated to have immunostimulatory activity in almost all three models of immunosuppression, PSMS was found to be more effi cacious against ethanol-induced immunosuppression when compared with pyrogallol induced immunosuppression and MTZ induced immunosuppression. However, dose-dependent improvement in immunological indices was evident in all three models. Conclusion: In summary, water soluble polysaccharides isolated from M. sagu stimulate the immunity in the animal models of immunosuppression.

Key words: Immunomodulation, immunosuppression, Metroxylon sagu, oxidative stress, polysaccharides.

}, keywords = {Immunomodulation, immunosuppression, Metroxylon sagu, Oxidative stress, Polysaccharides}, author = {Sireesha Pulla and Nagarjuna Sannithi and Siva Reddy Challa} }